Biosynthesis of Platinum Nanoparticles by Escherichia coli MC4100: Can Such Nanoparticles Exhibit Intrinsic Surface Enantioselectivity?
Biosynthesis of Platinum Nanoparticles by Escherichia coli MC4100: Can Such Nanoparticles Exhibit Intrinsic Surface Enantioselectivity?
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DOI：
10.1021/la204495z
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发表时间：
2012-03-20
期刊：
影响因子：
3.9
通讯作者：
Deplanche, Kevin
中科院分区：
文献类型：
--
作者：
Attard, Gary;Casadesus, Meritxell;Deplanche, Kevin
两种类型的铂生物纳米颗粒（bioNP）使用大肠杆菌MC 4100（1%和20%的金属负载质量）的生物制造，以及从细菌层释放纳米颗粒（NPs）和随后的临界清洗的方法的报告。以D-和L-葡萄糖电氧化为手性探针，研究了细菌制备的Pt纳米粒子表面形成对映体过量手性扭结位点的可能性。透射电子显微镜显示Pt bioNP（回收和清洁后）的直径通常为2.3 +/-0.7 nm（1%负载）和4.5 +/-0.7 nm（20%负载）。D-和L-葡萄糖电氧化测量使用Pt bioNP类型中的任一种都没有产生任何手性响应，但确实显示出不同的CV曲线。这表明，每个bioNP的整体表面形态可以通过金属负载的程度来控制，但不存在对映体过量的固有手性表面扭结位点。
The biomanufacture of two types of platinum bionanoparticle (bioNP) using Escherichia coli MC4100 (1% and 20% by mass metal loading) together with a method for both liberating the nanoparticles (NPs) from the bacterial layer and their subsequent critical cleaning is reported. The possibility of an enantiomeric excess of chiral kink sites forming on the surface of the Pt nanoparticles produced by the bacteria was investigated using the electrooxidation of D- and L-glucose as the chiral probe. Transmission electron microscopy revealed that the Pt bioNPs (after recovery and cleaning) were typically 2.3 +/- 0.7 nm (1% loading) and 4.5 +/- 0.7 nm (20% loading) in diameter. The D- and L-glucose electrooxidation measurements did not give rise to any chiral response using either of the Pt bioNPs types but did display differing CV profiles. This suggested that the overall surface morphology of each bioNP could be controlled by the degree of metal loading but that no enantiomeric excess of intrinsically chiral surface kink sites was present.