Gold-ATP
Gold-ATP
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DOI：
10.1006/jsbi.1999.4146
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发表时间：
1999-09-01
影响因子：
3
通讯作者：
Barcena, M
中科院分区：
文献类型：
--
作者：
Hainfeld, JF;Liu, WQ;Barcena, M
本文描述了ATP和十一金之间的两种共价偶联物的合成、纯化和化学分析，其中一种是金附着在ATP的核糖部分，另一种是金附着在腺嘌呤碱基的N-6位置。前一种探针随后被用于结合两种ATP结合蛋白，解旋酶dna和伴侣dna。用柱层析法纯化未结合的金后，用紫外-可见光谱法测定结合情况，然后用扫描透射电镜观察蛋白质和金的结合情况。偶联物发现，在没有ATP附着的十一金对照中几乎没有发生结合。该探针可用于研究蛋白质上的核苷酸结合位点或直接标记核酸或寡核苷酸。(C) 1999学术出版社。
The synthesis, purification, and chemical analysis of two covalent conjugates between ATP and undecagold are described, one in which gold is attached to the ribose moiety of ATP and the other in which it is attached to the N-6 position of the adenine base. The former probe was then used to bind to two ATP binding proteins, the helicase DnaB and the chaperone DnaK. After purification from unbound gold by column chromatography, binding was measured by UV-Vis spectroscopy, then the protein and gold were visualized by scanning transmission electron microscopy. finding was observed with the conjugates, and virtually no binding occurred in the control of undecagold without the ATP attached. This new probe may be useful for studying nucleotide binding sites on proteins or for labeling nucleic acids or oligonucleotides directly. (C) 1999 Academic Press.