Nano-hydroxyapatite in oral care cosmetics: characterization and cytotoxicity assessment
Nano-hydroxyapatite in oral care cosmetics: characterization and cytotoxicity assessment
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DOI：
10.1038/s41598-019-47491-z
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发表时间：
2019-07-30
影响因子：
4.6
通讯作者：
Fernandes, Maria H.
中科院分区：
文献类型：
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作者：
Coelho, Catarina C.;Grenho, Liliana;Fernandes, Maria H.
纳米羟基磷灰石已被用作口腔护理成分，被纳入几种产品中用于治疗牙齿过敏和牙釉质脱矿。尽管其结果令人鼓舞，但欧洲消费者安全科学委员会（SCCS）已就羟基磷灰石纳米颗粒在口腔护理产品中的使用讨论和质疑了监管和安全问题。在这项工作中，市售的纳米羟基磷灰石的特点和对人牙龈成纤维细胞的细胞相容性进行了评价，以及其刺激潜力，使用体外HET-CAM测定。本研究中选择的所有条件都试图模拟刷牙过程和通常掺入口腔护理产品中的羟基磷灰石纳米颗粒水平。在这项研究中使用的商业羟基磷灰石纳米粒子表现出棒状形态和预期的化学和相组成。体外细胞毒性参数显示，这些纳米颗粒对人牙龈成纤维细胞具有高度的细胞相容性。此外，这些纳米颗粒在HET-CAM测定中不具有任何刺激潜力。这项研究澄清了SCCS提出的问题，并得出结论，这种特定的纳米羟基磷灰石是细胞相容性的，因为这些纳米颗粒没有改变细胞的正常行为。因此，它们可以安全地用于口腔护理产品。
Nano-hydroxyapatite has been used as an oral care ingredient, being incorporated in several products for the treatment of dental hypersensitivity and enamel remineralisation. Despite its promising results, regulatory and safety concerns have been discussed and questioned by the European Scientific Committee on Consumer Safety (SCCS) regarding the usage of hydroxyapatite nanoparticles in oral care products. In this work, a commercially available nano-hydroxyapatite was characterized and its cytocompatibility towards human gingival fibroblasts was evaluated, as well as its irritation potential using the in vitro HET-CAM assay. All the conditions chosen in this study tried to simulate the tooth brushing procedure and the hydroxyapatite nanoparticles levels normally incorporated in oral care products. The commercial hydroxyapatite nanoparticles used in this study exhibited a rodlike morphology and the expected chemical and phase composition. The set of in vitro cytotoxicity parameters accessed showed that these nanoparticles are highly cytocompatible towards human gingival fibroblasts. Additionally, these nanoparticles did not possess any irritation potential on HET-CAM assay. This study clarifies the issues raised by SCCS and it concludes that this specific nano-hydroxyapatite is cytocompatible, as these nanoparticles did not alter the normal behaviour of the cells. Therefore, they are safe to be used in oral care products.