Delphinidin induced protective autophagy via mTOR pathway suppression and AMPK pathway activation in HER-2 positive breast cancer cells.
Delphinidin induced protective autophagy via mTOR pathway suppression and AMPK pathway activation in HER-2 positive breast cancer cells.
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飞燕草素通过抑制 mTOR 通路和激活 AMPK 通路在 HER-2 阳性乳腺癌细胞中诱导保护性自噬
DOI：
10.1186/s12885-018-4231-y
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发表时间：
2018-03-27
期刊：
影响因子：
3.8
通讯作者：
Yu X
中科院分区：
文献类型：
--
作者：
Chen J;Zhu Y;Zhang W;Peng X;Zhou J;Li F;Han B;Liu X;Ou Y;Yu X
背景我们之前已经证明了花青素的抗癌作用。本研究探讨了花青素单体中最常见的飞燕草素在 HER-2 阳性乳腺癌 MDA-MB-453 和 BT474 细胞中与自噬相关的生物活性。方法分析不同剂量飞燕草素对 MDA-MB-453 和 BT474 细胞增殖和凋亡的影响。自噬被认为是影响化疗的关键因素，并对飞燕草素处理的细胞中的自噬机制进行了研究。采用自噬抑制剂3-MA和BA1分析自噬抑制效果。结果飞燕草素对MDA-MB-453和BT474细胞具有抑制增殖、促进细胞凋亡、诱导自噬的作用，且呈剂量依赖性。在两种 HER-2 阳性乳腺癌细胞中，自噬的抑制增强了花翠素诱导的细胞凋亡和抗增殖作用。此外，飞燕草素通过抑制 HER-2 阳性乳腺癌细胞中的 mTOR 信号通路和激活 AMPK 信号通路诱导自噬。结论总的来说，结果表明飞燕草素诱导 HER-2 阳性乳腺癌细胞凋亡和自噬，并且自噬是通过 mTOR 和 AMPK 信号通路诱导的。自噬的抑制促进了飞燕草素的抗癌作用。
BackgroundWe have previously demonstrated the anticancer effect of anthocyanins. In this study, we explored the biological activities of delphinidin, the most common of the anthocyanidin monomers, that were related to autophagy in HER-2 positive breast cancer MDA-MB-453 and BT474 cells.MethodsThe effects of various doses of delphinidin on the proliferation and apoptosis of MDA-MB-453 and BT474 cells were analysed. Autophagy was identified as a critical factor that influenced chemotherapy, and the autophagic mechanism in delphinidin-treated cells was investigated. The autophagy inhibitors, 3-MA and BA1, were used to analyse the effects of autophagy inhibition.ResultsDelphinidin inhibited proliferation, promoted apoptosis, and induced autophagy in MDA-MB-453 and BT474 cells in a dose-dependent manner. The inhibition of autophagy enhanced the delphinidin-induced apoptosis and antiproliferative effect in both HER-2 positive breast cancer cells. In addition, delphinidin induced autophagy via suppression of the mTOR signalling pathway and activation of the AMPK signalling pathway in HER-2 positive breast cancer cells.ConclusionsCollectively, the results showed that delphinidin induced apoptosis and autophagy in HER-2 positive breast cancer cells and that autophagy was induced via the mTOR and AMPK signalling pathways. The suppression of autophagy promoted the anticancer effects of delphinidin.
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影响因子：
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