Laboratory Transmission of Japanese Encephalitis and West Nile Viruses by Molestus Form of Culex pipiens (Diptera: Culicidae) Collected in Uzbekistan in 2004
Laboratory Transmission of Japanese Encephalitis and West Nile Viruses by Molestus Form of Culex pipiens (Diptera: Culicidae) Collected in Uzbekistan in 2004
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2004 年乌兹别克斯坦采集的淡色库蚊（双翅目：库蚊科）所致日本脑炎和西尼罗河病毒的实验室传播
DOI：
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发表时间：
2006
影响因子：
2.1
通讯作者：
S. Khodjaev
中科院分区：
文献类型：
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作者：
M. Turell;C. Mores;D. Dohm;N. Komilov;J. Paragas;John S. Lee;D. Shermuhemedova;T. Endy;A. Kodirov;S. Khodjaev
摘要本文对淡色库蚊骚扰型进行了评价。（以下称为“骚扰者”）在实验室条件下传播日本脑炎（黄病毒科，黄病毒属，JEV）和西尼罗河（黄病毒科，黄病毒属，WNV）病毒的能力。这些骚扰者是西尼罗河病毒的高能力实验室载体，感染率和传播率分别为96%和81%。大约75%的女性性骚扰者在传播感染后进食，通过叮咬传播病毒。因此，预计在感染性血餐后16至25天之间服用第二次血餐的骚扰者中，约有60%会通过叮咬传播西尼罗河病毒。相比之下，这些骚扰者是JEV的效率较低的载体，感染率和传播率分别为51%和25%。此外，只有33%的播散性感染者通过叮咬传播JEV，表明存在显著的唾液腺屏障。因此，如果在16-25天后服用第二次血餐，预计只有0.8%的口服暴露个体会通过叮咬传播JEV。这些数据表明，骚扰形式的Cx。p. pipiens应被视为乌兹别克斯坦西尼罗河病毒的潜在重要载体，如果这种病毒传入乌兹别克斯坦，可能参与JEV的传播。
Abstract We evaluated the molestus form of Culex pipiens pipiens (L.) (hereafter referred to as “molestus”) captured near Tashkent, Uzbekistan, for their ability to transmit Japanese encephalitis (family Flaviviridae, genus Flavivirus, JEV) and West Nile (family Flaviviridae, genus Flavivirus, WNV) viruses under laboratory conditions. These molestus were highly competent laboratory vectors of WNV, with infection and dissemination rates of 96 and 81%, respectively. Approximately 75% of female molestus that fed after development of a disseminated infection transmitted virus by bite. Therefore, ≈60% of those molestus taking a second bloodmeal between 16 and 25 d after an infectious bloodmeal would be expected to transmit WNV by bite. In contrast, these molestus were less efficient vectors of JEV, with infection and dissemination rates of 51 and 25%, respectively. In addition, only 33% of individuals with a disseminated infection transmitted JEV by bite, indicating a significant salivary gland barrier. Therefore, only ≈8% of orally exposed individuals would be expected to transmit JEV by bite if they took a second bloodmeal 16–25 d later. These data indicate that the molestus form of Cx. p. pipiens should be considered a potentially important vector of WNV in Uzbekistan and may become involved in the transmission of JEV, should this virus be introduced into Uzbekistan.