The influence of the enzyme membrane thickness on the response of amperometric biosensors
The influence of the enzyme membrane thickness on the response of amperometric biosensors
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DOI：
10.3390/s30700248
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发表时间：
2003-07-01
期刊：
影响因子：
3.9
通讯作者：
Kulys, J
中科院分区：
文献类型：
--
作者：
Baronas, R;Ivanauskas, F;Kulys, J
建立了安培生物传感器的数学模型。该模型基于非平稳扩散方程，其中包含与酶促反应的Michaelis-Menten动力学相关的非线性项。采用数字模拟的方法，研究了酶膜厚度对生物传感器响应的影响。生物传感器操作的数字模拟显示，在不同的最大酶速率下，最大生物传感器电流随膜厚度的变化是非单调的。采用有限差分技术进行了数字仿真。数值模拟结果与已知解析解进行了比较。本文提出了一个选择膜厚度的框架，以确保生物传感器在最大酶速率的所需范围内具有足够稳定的灵敏度。
A mathematical model of amperometric biosensors has been developed. The model is based on non-stationary diffusion equations containing a non-linear term related to Michaelis-Menten kinetics of the enzymatic reaction. Using digital simulation, the influence of the thickness of enzyme membrane on the biosensor response was investigated. The digital simulation of the biosensor operation showed the non-monotonous change of the maximal biosensor current versus the membrane thickness at the various maximal enzymatic rates. Digital simulation was carried out using the finite difference technique. Results of the numerical simulation was compared with known analytical solutions. This paper presents a framework for selection of the membrane thickness, ensuring the sufficiently stable sensitivity of a biosensor in a required range of the maximal enzymatic rate.