Effects of bright light exposure during daytime on peripheral clock gene expression in humans.
Effects of bright light exposure during daytime on peripheral clock gene expression in humans.
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白天强光照射对人类外周时钟基因表达的影响。
DOI：
10.1007/s00484-016-1294-9
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Sato Motohiko
中科院分区：
文献类型：
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作者：
Sato Maki;Wakamura T;Morita T;Okamoto A;Akashi M;Matsui T;Sato Motohiko
光是控制昼夜节律的最强同步器。光的强度和持续时间全年都在变化，从而影响人类和动物的体重、食物偏好和褪黑素分泌。尽管生物钟基因的表达已经用人类样本进行了检测，但目前尚不清楚白天的强光是否会影响人类生物钟基因的表达。因此，我们在此研究了白天强光照射对人类头皮毛囊和根细胞中时钟基因表达的影响。健康男性7名，年龄20.4±2.2岁，身高172.3±5.8 cm，体重64.3±8.5 kg, BMI 21.7±3.1 kg/m2, mean±SD。受试者在一个月内完成两次为期3天的实验，分别暴露在明亮和昏暗的光线条件下。采用支链DNA探针检测per1 - 3、Cry1-2、Rev-erb-α(Nr1d1)、Rev-erb-β(Nr1d2)和dec1的mrna表达。在强光条件下，per1、Per2、Per3、Cry1、Cry2、rev - erbb -α(Nr1d1)、dec1的表达未见明显变化。而rev - erbb -β(Nr1d2)在强光条件下的表达强于弱光条件。这些结果表明，强光刺激不会影响人类生物钟基因的表达。白天长时间的强光照射可能需要改变人类生物钟基因的表达。
Light is the strongest synchronizer controlling circadian rhythms. The intensity and duration of light change throughout the year, thereby influencing body weight, food preferences, and melatonin secretion in humans and animals. Although the expression of clock genes has been examined using human samples, it currently remains unknown whether bright light during the daytime affects the expression of these genes in humans. Therefore, we herein investigated the effects of bright light exposure during the daytime on clock gene expression in the hair follicular and root cells of the human scalp. Seven healthy men (20.4 ± 2.2 years old; 172.3 ± 5.8 cm; 64.3 ± 8.5 kg; BMI 21.7 ± 3.1 kg/m2, mean ± SD) participated in this study. Subjects completed 3-day experimental sessions twice in 1 month during which they were exposed to bright and dim light conditions. ThemRNAexpression ofPer1–3,Cry1–2,Rev-erb-α(Nr1d1),Rev-erb-β(Nr1d2), andDec1was analyzed using branched DNA probes. No significant changes were observed in the expression ofPer1,Per2,Per3,Cry1,Cry2,Rev-erb-α(Nr1d1), orDec1following exposure to bright light conditions. However, the expression ofRev-erb-β(Nr1d2) tended to be stronger under bright light than dim light conditions. These results suggest that the bright light stimulus did not influence the expression of clock genes in humans. Long-lasting bright light exposure during the daytime may be required to change the expression of clock genes in humans.