Various abiotic stresses vapidly activate Arabidopsis MAP kinases ATMPK4 and ATMPK6
Various abiotic stresses vapidly activate Arabidopsis MAP kinases ATMPK4 and ATMPK6
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DOI：
10.1046/j.1365-313x.2000.00913.x
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发表时间：
2000-12-01
期刊：
影响因子：
7.2
通讯作者：
Shinozaki, K
中科院分区：
文献类型：
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作者：
Ichimura, K;Mizoguchi, T;Shinozaki, K
丝裂原活化蛋白激酶（Mitogen-activated protein kinase，MAP kinase，MAPK）级联反应在多种生物体的细胞外刺激（如环境胁迫和生长调节剂）的信号转导中起着关键作用。拟南芥MAP激酶构成一个基因家族，但每个MAP激酶的刺激信号尚未阐明。在这里，我们表明，环境压力，如低温，低湿度，高渗透压，触摸和创伤诱导拟南芥MAP激酶ATMPK 4和ATMPK 6的快速和短暂的激活。ATMPK 4和ATMPK 6的激活与酪氨酸磷酸化相关，但与mRNA或蛋白质的量无关。这两种MAP激酶在活化过程中的动力学不同。这些结果表明，ATMPK 4和ATMPK 6参与不同的信号转导途径，响应这些环境压力。
Mitogen-activated protein kinase (MAP kinase, MAPK) cascades play pivotal roles in signal transduction of extracellular stimuli, such as environmental stresses and growth regulators, in various organisms. Arabidopsis thaliana MAP kinases constitute a gene family, but stimulatory signals for each MAP kinase have not been elucidated. Here we show that environmental stresses such as low temperature, low humidity, hyper-osmolarity, touch and wounding induce rapid and transient activation of the Arabidopsis MAP kinases ATMPK4 and ATMPK6. Activation of ATMPK4 and ATMPK6 was associated with tyrosine phosphorylation but not with the amounts of mRNA or protein. Kinetics during activation differ between these two MAP kinases. These results suggest that ATMPK4 and ATMPK6 are involved in distinct signal transduction pathways responding to these environmental stresses.