Regulatory and functional interactions between ovarian tumor and ovo during Drosophila oogenesis
Regulatory and functional interactions between ovarian tumor and ovo during Drosophila oogenesis
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DOI：
10.1016/s0925-4773(99)00167-7
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发表时间：
1999-10-01
影响因子：
2.6
通讯作者：
Nagoshi, RN
中科院分区：
文献类型：
--
作者：
Hinson, S;Pettus, J;Nagoshi, RN
卵和卵巢肿瘤基因在果蝇卵子发生的早期和晚期都是必需的。卵产物是一种锌指转录因子，可以结合到卵巢肿瘤启动子的位点并影响其表达水平。我们对卵零突变细胞器的检查表明，除了已知的维持生殖细胞数量的作用之外，它还需要在幼虫生殖阶段XX生殖细胞的分化。相反，在蛹期和成虫期，卵囊细胞的分裂需要卵巢的分泌，从而产生卵室。对性转化果蝇的研究表明，卵和卵巢肿瘤无效突变表型与雄性生殖细胞在雌性索马中发育时产生的生殖系缺陷不同，并且比后者更严重。这表明卵细胞和卵巢肿瘤除了在生殖细胞性别决定中的作用外，还具有促卵功能。我们还表明，卵巢肿瘤的调节卵是阶段特异性的，卵巢肿瘤启动子的活性不需要卵在幼虫阶段，但成为卵依赖于成年卵巢。这与卵巢肿瘤启动子对来自索马的性别特异性信号的反应一致，表明卵子发生期间卵巢肿瘤的体细胞和生殖细胞调节途径的会聚。(C)1999爱思唯尔科学爱尔兰有限公司保留所有权利。
The ovo and ovarian tumor genes are required during early and late stages of Drosophila oogenesis. The ovo product, a zinc-finger transcription factor, can bind to sites and influence the level of expression of the ovarian tumor promoter. Our examination of ovo null mutant organelles demonstrate that it is required for the differentiation of XX germ cells during larval genial stages, in addition to its known role in maintaining germ cell numbers. In contrast, ovarian amor is required during pupal and adult stages for the cystocyte divisions that give rise to the egg chamber. Studies on sexually transformed flies indicate that both the ovo and ovarian tumor null mutant phenotypes are distinctive from and more severe than the germline defects produced when male germ cells develop in female soma. This suggests that ovo and ovarian tumor have oogenic functions other than their putative role in germline sex determination. We also demonstrate that the regulation of ovarian tumor by ovo is stage-specific, as ovarian tumor promoter activity does not require ovo during larval stages but becomes ovo-dependent in the adult ovary. This coincides with when the ovarian tumor promoter becomes responsive to sex-specific signals from the soma suggesting a convergence of somatic and germline regulatory pathways on ovarian tumor during oogenesis. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.