Interleukin 1 production by human polymorphonuclear neutrophils.
Interleukin 1 production by human polymorphonuclear neutrophils.
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由人多形核中性粒细胞产生白细胞介素 1。
DOI：
10.4049/jimmunol.136.10.3677
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发表时间：
1986
影响因子：
4.4
通讯作者：
J. Skosey
中科院分区：
文献类型：
--
作者：
K. Tiku;M. Tiku;J. Skosey
本研究的目的是确定是否人类多形核中性粒细胞（PMN），这与巨噬细胞共享一个共同的细胞谱系，可以产生因子，如IL 1。这两种细胞类型共有的其他特性是它们的吞噬性质和它们细胞表面上的共同受体和抗原。IL 1在许多物理、生化和功能特性上与内源性热原（EP）相似。中性粒细胞被认为是EP的一种可能的细胞来源，但也有报道质疑中性粒细胞产生EP的能力。该研究表明，正常人PMN可以被颗粒剂如酵母聚糖和可溶性剂如佛波醇肉豆蔻乙酸酯刺激，以产生诱导小鼠胸腺细胞增殖的因子，即，PMN IL 1. PMN释放IL 1呈剂量和时间依赖性。中性粒细胞IL 1是不可透析的，是热不稳定的，并在pH值低于5和高于8时失活。PMN IL 1刺激正常人滑膜成纤维细胞增殖，并引起滑膜细胞释放中性蛋白酶（纤溶酶原激活物）。蛋白酶抑制剂抑肽酶或大豆胰蛋白酶抑制剂均不影响PMN IL 1的滑膜和胸腺细胞增殖能力。凝胶过滤研究估计分子量。中性粒细胞白细胞介素1大约是13,000到17000。
The purpose of this study was to determine whether human polymorphonuclear neutrophils (PMN), which share a common cell lineage with macrophages, could produce factors such as IL 1. Other properties which these two cell types share are their phagocytic nature and the common receptor and antigens on their cell surfaces. IL 1, in many of its physical, biochemical, and functional characteristics, is found to resemble endogenous pyrogen (EP). PMN have been cited as a possible cell source of EP, but there have also been reports in which the capacity of PMN to produce EP has been questioned. This study shows that normal human PMN can be stimulated by particulate agents such as zymosan and soluble agents such as phorbol myristic acetate to produce a factor(s) which induces proliferation of mouse thymocytes, i.e., PMN IL 1. This PMN IL 1 was released from PMN in a dose- and time-dependent fashion. PMN IL 1 was nondialyzable, was heat-labile, and was inactivated at pH below 5 and above 8. PMN IL 1 stimulated the proliferation of normal human synovial fibroblasts and caused release of a neutral protease (plasminogen activator) from synovial cells. The synovial and thymocyte-proliferating capacity of PMN IL 1 was not affected by the protease inhibitor aprotinin or by soybean trypsin inhibitor. Gel filtration studies estimate the m.w. of PMN IL 1 to be approximately 13,000 to 17,000.