EXAMINATION OF SURVIVAL, PROLIFERATION AND CELL-SURFACE ANTIGEN EXPRESSION OF HUMAN MONOCYTES EXPOSED TO MACROPHAGE COLONY-STIMULATING FACTOR (M-CSF)
EXAMINATION OF SURVIVAL, PROLIFERATION AND CELL-SURFACE ANTIGEN EXPRESSION OF HUMAN MONOCYTES EXPOSED TO MACROPHAGE COLONY-STIMULATING FACTOR (M-CSF)
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DOI：
10.1002/stem.5530080503
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发表时间：
1990-09-01
期刊：
影响因子：
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通讯作者：
ABBOUD, CN
中科院分区：
文献类型：
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作者：
ERICKSONMILLER, CL;BRENNAN, JK;ABBOUD, CN
观察巨噬细胞集落刺激因子(M-CSF)或巨噬细胞集落刺激因子-1(CSF-1)对人单核细胞存活、增殖、成熟及活化的影响。M-CSF(100-1000U/ml)可使培养8天后存活的单核细胞数增加一倍，并加速细胞体积的增加。M-CSF的抗血清可消除这两种作用。在这段时间内，单核细胞的~3H-胸腺嘧啶核苷掺入没有显著增加。在所测试的各种因素中，包括γ-干扰素(γ-干扰素)、白介素1α、粒细胞集落刺激因子(G-CSF)、血小板衍生生长因子(PDGF)和脂多糖(LPS)，只有粒-巨噬细胞集落刺激因子(GM-CSF)也能提高存活率和增加细胞体积。人M-CSF抗血清可消除GM-CSF诱导的小鼠存活增加，但不能消除GM-CSF刺激的单核细胞体积的增加。单核细胞表面标记随着成熟(即Fc.Gamma.RIII)或激活(即Fc.Gamma.RI)的增加而增加，不受M-CSF孵育的影响。
The effects of macrophage colony-stimulating factor (M-CSF or CSF-1) on the survival, proliferation, maturation and activation of human blood monocytes were examined. M-CSF (100-1,000 U/ml) doubled the number of monocytes surviving after eight days in culture and accelerated the usual increase in cell volume. Antiserum to M-CSF abolished both of these effects. There was no sizable increase in 3H-thymidine incorporation in monocytes over this time period. Of various factors tested, including .gamma.-interferon (.gamma.-IFN), interleukin (IL) 1.alpha., granulocyte CSF (G-CSF), platelet- derived growth factor (PDGF), and lipopolysaccharide (LPS), only granulocyte-macrophage CSF (GM-CSF) could also enhance survival and augment cell volume. While antiserum to human M-CSF eliminated the increase in survival induced by GM-CSF, it could not ablate the GM-CSF-stimulated increase in monocyte cell volume. Monocyte cell surface markers that increase with maturation (i.e., Fc.gamma.RIII) or with activation (i.e., Fc.gamma.RI) were unaffected by incubation with M-CSF.