Carbon Dots Enable Efficient Delivery of Functional DNA in Plants.
Carbon Dots Enable Efficient Delivery of Functional DNA in Plants.
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DOI：
10.1021/acsabm.0c01170
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发表时间：
2020-11
影响因子：
4.7
通讯作者：
Bo Wang;Jian Huang;Mengling Zhang;Yun Wang;Huibo Wang;Yurong Ma;Xiaodong Zhao;Xiao Wang;Changhong Liu;Hui Huang;Yang Liu;Fang Lu;Hengxiu Yu;Mingwang Shao;Zhenhui Kang
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文献类型：
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作者：
Bo Wang;Jian Huang;Mengling Zhang;Yun Wang;Huibo Wang;Yurong Ma;Xiaodong Zhao;Xiao Wang;Changhong Liu;Hui Huang;Yang Liu;Fang Lu;Hengxiu Yu;Mingwang Shao;Zhenhui Kang
基因工程是植物科学中的一项重要技术。目前的基因转移技术，如农杆菌介导的基因转移和基因枪瞬时表达系统等，往往需要非常复杂的过程，或者由于细胞壁结构的抑制而具有宿主范围的局限性。在这里，我们证明了通过将聚乙烯亚胺（PEI）与碳点（CD）相结合制备的纳米复合材料（CDP）被证实是一种很好的载体，用于动物和植物细胞中的基因转染，特别是在完整的植物中。在水稻（Oryza Sativa japonica）、小麦（Triticum aestivum）和绿豆（Phaseolus radiatus）的叶和水稻根中实现了高效的DNA递送和强基因表达而没有DNA整合。值得注意的是，CDP成功地将潮霉素抗性基因导入水稻中并在水稻中诱导潮霉素抗性。CDP可实现β-葡萄糖醛酸酶基因在水稻成熟胚诱导的愈伤组织中的传递和表达。CDP不仅促进质粒转运到细胞中，而且保护DNA免受DNA酶降解。我们的工作提供了一个高效的基因表达载体系统，这可能是一个有用的和快速的基因工程工具，在植物生物学的功能研究。
Genetic engineering is an important technology in plant sciences. The current delivery techniques, such as Agrobacterium-mediated gene transfer and gene gun instant gene expression system, always need a very complicated process or have host-range limitations due to the inhibition of the cell wall structure. Here, we demonstrate that a nanocomposite (CDP) prepared by combining polyethyleneimine (PEI) with carbon dot (CD) was confirmed as a good vector for gene transfection in both animal and plant cells, especially in intact plants. Efficient DNA delivery and strong gene expression without DNA integration are accomplished in rice (Oryza Sativa japonica), wheat (Triticum aestivum), and mung bean (Phaseolus radiatus) leaves and rice roots. Notably, the CDP successfully deliver the hydamycin resistance gene into the rice and induce hygromycin resistance in rice. Also, CDP can achieve the β-glucuronidase gene delivery and expression in mature rice embryo induced callus. The CDP not only facilitates plasmid transport into cells but also protects DNA from DNase degradation. Our work provides a highly efficient DNA delivery system for instant gene expression, which could be a useful and fast gene engineering tool for functional research in plant biology.