Characterization of a promoter within the first intron of the human CD4 gene.
Characterization of a promoter within the first intron of the human CD4 gene.
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人类 CD4 基因第一个内含子内启动子的表征。
DOI：
10.1111/j.1432-1033.1997.00768.x
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Neudorf,SM
中科院分区：
文献类型：
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作者：
Rushton,JJ;Zorich,GP;Stolc,V;Neudorf,SM
CD4分子在T细胞分化和活化过程中受到复杂的调节。调节CD 4基因表达的元件仅部分被确定。在本报告中，我们鉴定了位于CD 4基因第一内含子的启动子元件。该启动子优先在T细胞系中起作用，并且优先在CD4+、CD8+细胞中有活性。这些发现类似于其他系统，其中多个启动子定义组织和发育特异性表达模式。通过一系列的缺失，电泳迁移率变动分析和核酸外切酶III保护分析，我们将基础启动子元件定位到32 bp片段。该元件缺乏rnyb和ets的潜在结合结构域，这两者先前已被证明参与5′鼠和人CD4启动子的功能，这表明存在一种新的T细胞特异性转录因子。这些结果也表明，CD4的表达需要使用位于整个CD4基因的多个调控元件。
The CD4 molecule is subject to complex regulation during T cell differentiation and activation. The elements regulatingCD4gene expression have only partially been defined. In this report, we identified a promoter element located in the first intron of theCD4gene. This promoter preferentially functions in T cell lines and is preferentially active in CD4+, CD8+ cells. These findings are similar to other systems in which multiple promoters define tissue‐ and developmental‐specific patterns of expression. Through a series of deletions, electrophoretic mobility shift assays and exonuclease III protection assays, we localized the basal promoter element to a 32‐bp fragment. This element lacks potential binding domains for rnyb and ets, both of which have previously been shown to be involved in the function of the 5′ murine and human CD4 promoter, and this suggests the presence of a novel, T‐cell‐specific transcription factor. These results also suggest that theCD4expression requires the use of multiple regulatory elements located throughout theCD4gene.