Mechanics of leukocyte deformation and adhesion to endothelium in shear flow
Mechanics of leukocyte deformation and adhesion to endothelium in shear flow
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DOI：
10.1114/1.143
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发表时间：
1999-05-01
影响因子：
3.8
通讯作者：
Lipowsky, HW
中科院分区：
文献类型：
--
作者：
Dong, C;Cao, J;Lipowsky, HW
本文研究了白细胞（白色细胞）在剪切力作用下变形和粘附于内皮细胞（EC）的机理。从体内测量获得了瞬时WBC-EC粘附的实验数据。WBC-EC接触的显微图像显示，在初始WBC滚动，对于一个给定的壁剪切应力，接触面积随着时间的推移，新的债券形成在前缘，然后随着时间的推移，随着WBC膜的后缘剥离远离EC减少。一个二维模型（2D）开发的弹性环粘附到流体应力下的表面组成。该环代表富含肌动蛋白的WBC皮质层，并包含不可压缩的流体作为细胞内部。所有的分子键被建模为弹性弹簧分布在WBC-EC接触区域。WBC附近的壁面剪切应力（tau（w））与产生的拖曳力（F-s）之间的比例变化，即，F-s/tau（w）显示其随WBC变形和血管通道高度增加而降低（2D）。计算还发现粘附的WBC和EC之间的剥离区可能占总接触界面的不到5%。计算研究描述了WBC-EC粘附和粘附过程中WBC变形的程度。(C)1999年生物医学工程学会。[S0090-6964（99）01103-0]。
The mechanics of leukocyte [white blood cell (WBC)] deformation and adhesion to endothelial cells (EC) in shear how has been investigated. Experimental data an transient WBC-EC adhesion were obtained from in vivo measurements. Microscopic images of WBC-EC contact during incipient WBC rolling revealed that for a given wall shear stress, the contact area increases with time as new bonds are formed at the leading edge, and then decreases with time as the trailing edge of the WBC membrane peels away from the EC. A two-dimensional model (2D) was developed consisting of an elastic ring adhered to a surface under fluid stresses. This ring represents an actin-rich WBC cortical layer and contains an incompressible fluid as the cell interior. All molecular bonds are modeled as elastic springs distributed in the WBC-EC contact region. Variations of the proportionality between wall shear stress (tau(w)) in the vicinity of the WBC and the resulting drag force (F-s), i.e., F-s/tau(w), reveal its decrease with WBC deformation and increasing vessel channel height (2D). The computations also find that the peeling zone between adherent WBC and EC may account for less than 5% of the total contact interface. Computational studies describe the WBC-EC adhesion and the extent of WBC deformation during the adhesive process. (C) 1999 Biomedical Engineering Society. [S0090-6964(99)01103-0].