Synthesis of biologically active biotinylated muramyl dipeptides
Synthesis of biologically active biotinylated muramyl dipeptides
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DOI：
10.1016/j.bmcl.2010.08.056
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发表时间：
2010-10-15
影响因子：
2.7
通讯作者：
O'Shea, Erin K.
中科院分区：
文献类型：
--
作者：
Grimes, Catherine Leimkuhler;Podolsky, Daniel K.;O'Shea, Erin K.
胞壁酰二肽（MDP）被认为与先天免疫受体Nod2相互作用。为了鉴定MDP的细胞受体，我们合成了生物素化的MDP异构体，并在基于细胞的测定中测试了这些化合物激活Nod2的能力。我们发现MDP的修饰不会干扰其激活Nod2的能力。这些标记形式的MDP将用于鉴定免疫刺激分子的细胞受体。(c)2010爱思唯尔有限公司版权所有。
Muramyl dipeptide (MDP) is believed to interact with an innate immune receptor, Nod2. To identify the cellular receptor for MDP, we have synthesized biotinylated MDP isomers and tested the ability of these compounds to activate Nod2 in a cell-based assay. We found that the modification of MDP does not perturb its ability to activate Nod2. These tagged versions of MDP will be useful to identify the cellular receptor of the immunostimulatory molecules. (c) 2010 Elsevier Ltd. All rights reserved.