Water-Soluble Silicon Quantum Dots toward Fluorescence-Guided Photothermal Nanotherapy
Water-Soluble Silicon Quantum Dots toward Fluorescence-Guided Photothermal Nanotherapy
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DOI：
10.1021/acs.langmuir.1c02326
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发表时间：
2022-01-27
期刊：
影响因子：
3.9
通讯作者：
Shirahata, Naoto
中科院分区：
文献类型：
--
作者：
Ozbilgin, Irem Nur Gamze;Yamazaki, Tomohiko;Shirahata, Naoto
我们报道了端羧基硅量子点(SiQD)，由于表面单分子层的高分子覆盖率，具有高光致发光量子产率(PLQY)的明亮发光，用于监测电池的发光特性的长期稳定性，对电池的毒性较小，以及高的光热响应，因此在水中表现出高的溶解性。我们通过10-十一烯酸在氢端的硅氢化反应，在pH值为3的三乙氧基硅烷的热歧化反应和随后的氢氟酸刻蚀作用下，制备了水溶性的硅量子点(UA：SiQds)。我们通过细胞摄取、短期毒性和首次的长期细胞毒性来评估它们与活细胞的相互作用。结果表明，UA：SiQD具有良好的光学性质，能够成像超过18天，光热响应具有25.1%的光热转换效率，以及激光照射导致细胞死亡的直接证据，是治疗的潜在候选材料。UA：硅量子点具有较低的细胞毒性，短期内细胞存活率高达400微克/毫升，在50微克/毫升的浓度下孵育14天后细胞存活率为50%。
We report carboxy-terminated silicon quantum dots(SiQDs) that exhibit high solubility in water due to the high molecularcoverage of surface monolayers, bright light emission with highphotoluminescence quantum yields (PLQYs), long-term stability inthe PL property for monitoring cells, less toxicity to the cells, and a highphotothermal response. We prepared water-soluble SiQDs by thethermal hydrosilylation of 10-undecenoic acid on their hydrogen-terminated surfaces, provided by the thermal disproportionation oftriethoxysilane hydrolyzed at pH 3 and subsequent hydrofluoric etching.The 10-undecanoic acid-functionalized SiQDs (UA:SiQDs) showedlong-term stability in hydrophilic solvents including ethanol and water(pH 7). We assess their interaction with live cells by means of cellularuptake, short-term toxicity, and, for thefirst time, long-term cytotoxicity.Results show that UA:SiQDs are potential candidates for theranostics,with their good optical properties enabling imaging for more than 18 days and a photothermal response having a 25.1%photothermal conversion efficiency together with the direct evidence of cell death by laser irradiation. UA:SiQDs have lowcytotoxicity with full viability of up to 400 mu g/mL for the short term and a 50% cell viability value after 14 days of incubation at a 50 mu g/mL concentration