Compartmentalized nodes control mitotic entry signaling in fission yeast.
Compartmentalized nodes control mitotic entry signaling in fission yeast.
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DOI：
10.1091/mbc.e13-02-0104
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发表时间：
2013-06
影响因子：
3.3
通讯作者：
Moseley JB
中科院分区：
文献类型：
--
作者：
Deng L;Moseley JB
The conserved fission yeast protein Skb1 inhibits mitotic entry through the SAD kinase Cdr1. Skb1 and Cdr1 are spatially segregated in discrete sets of stable nodes at the cell cortex. This work reveals new spatial control elements of the mitotic size control system. Cell cycle progression is coupled to cell growth, but the mechanisms that generate growth-dependent cell cycle progression remain unclear. Fission yeast cells enter into mitosis at a defined size due to the conserved cell cycle kinases Cdr1 and Cdr2, which localize to a set of cortical nodes in the cell middle. Cdr2 is regulated by the cell polarity kinase Pom1, suggesting that interactions between cell polarity proteins and the Cdr1-Cdr2 module might underlie the coordination of cell growth and division. To identify the molecular connections between Cdr1/2 and cell polarity, we performed a comprehensive pairwise yeast two-hybrid screen. From the resulting interaction network, we found that the protein Skb1 interacted with both Cdr1 and the Cdr1 inhibitory target Wee1. Skb1 inhibited mitotic entry through negative regulation of Cdr1 and localized to both the cytoplasm and a novel set of cortical nodes. Skb1 nodes were distinct structures from Cdr1/2 nodes, and artificial targeting of Skb1 to Cdr1/2 nodes delayed entry into mitosis. We propose that the formation of distinct node structures in the cell cortex controls signaling pathways to link cell growth and division.
