Serum miR-21 may be a Potential Diagnostic Biomarker for Diabetic Nephropathy
Serum miR-21 may be a Potential Diagnostic Biomarker for Diabetic Nephropathy
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DOI：
10.1055/s-0035-1565095
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发表时间：
2016-07-01
影响因子：
1.8
通讯作者：
Yang, J.
中科院分区：
文献类型：
--
作者：
Wang, J.;Duan, L.;Yang, J.
miRNAs在许多病理过程的发生和发展中起重要作用。miR-21与糖尿病肾病（DN）密切相关。然而，血清miR-21是否可作为DN的潜在诊断标志物以及血清miR-21与组织miR-21的关系尚不清楚。本研究分别采用实时荧光定量RT-PCR、细胞转染、荧光素酶报告基因检测、蛋白质印迹和共聚焦显微镜等技术。在此，我们发现随着DN的进展，血清和肾组织中miR-21显著升高。荧光素酶报告基因检测显示smad 7是一个有效的miR-21靶点，细胞转染显示miR-21过表达下调靶点smad 7的表达。有趣的是，血清miR-21与组织miR-21的变化在DN的发展中显著一致。此外，血清miR-21与GBM、GA、ACR和CCF呈正相关，与Ccr呈负相关。重要的是，EscheromiR-21不仅减轻GBM、GA、ACR和CCF，而且通过增加靶Smad 7改善Ccr。结论：血清miR-21与DN的肾脏结构和功能密切相关，血清miR-21可作为DN的潜在诊断生物标志物。
MiRNAs play important roles in initiation and progress of many pathologic processes. MiR-21 was closely associated with diabetic nephropathy (DN). However, whether serum miR-21 was as a potential diagnostic biomarker for DN and the relationship between serum miR-21 and tissue miR-21 remained unclear. In this study, real-time RT-PCR, cell transfection, luciferase reporter gene assays, western blot and confocal microscope were used, respectively. Here, we found that serum and renal tissue miR-21 was significantly elevated with the progress of DN. Moreover, luciferase reporter gene assays showed that smad7 was a validated miR-21 target, cell transfection showed that miR-21 overexpression downregulated target smad7 expression. Interestingly, serum miR-21 was significantly consistent with the alterations of tissue miR-21 with the development of DN. In addition, serum miR-21 was also positively correlated with GBM, GA, ACR and CCF, while negatively correlated with Ccr. Importantly, antagomiR-21 not only alleviated GBM, GA, ACR and CCF, but also ameliorated Ccr by increasing target smad7. In conclusion, our data demonstrated that serum miR-21 was closely associated with renal structure and function, and serum miR-21 may be regarded as a potential diagnostic biomarker of DN.