GFP-tagged balancer chromosomes for Drosophila melanogaster
GFP-tagged balancer chromosomes for Drosophila melanogaster
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DOI：
10.1016/s0925-4773(00)00248-3
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发表时间：
2000-03-01
影响因子：
2.6
通讯作者：
Kornberg, Thomas B.
中科院分区：
文献类型：
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作者：
Casso, David;Ramirez-Weber, Felipe-Andres;Kornberg, Thomas B.
我们为黑腹果蝇的三条主要染色体分别构建了表达绿色荧光蛋白（GFP）的平衡染色体。GFP在这些染色体中的表达是由Kruppel （Kr）启动子通过酵母GAL4-UAS调控系统间接驱动的。GFP荧光早在胚带伸展期就可以在胚胎中看到，在幼虫、蛹和成虫中也可以看到。我们展示了这些平衡子的GFP表达模式，并展示了使用平衡子来识别纯合后代。
We constructed green fluorescent protein (GFP)-expressing balancer chromosomes for each of the three major chromosomes of Drosophila melanogaster. Expression of GFP in these chromosomes is driven indirectly by a Kruppel (Kr) promoter, via the yeast GAL4-UAS regulatory system. GFP fluorescence can be seen in embryos as early as the germ band extension stage, and can also be seen in larvae, pupae, and adults. We show the patterns of GFP expression of these balancers and demonstrate the use of the balancers to identify homozygous progeny.