BDNF expression in olfactory bulb and epithelium during regeneration of olfactory epithelium.
BDNF expression in olfactory bulb and epithelium during regeneration of olfactory epithelium.
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嗅上皮再生过程中嗅球和上皮中的 BDNF 表达。
DOI：
10.1016/j.neulet.2012.03.051
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发表时间：
2012
影响因子：
2.5
通讯作者：
Nibu K.
中科院分区：
文献类型：
--
作者：
Uranagase A;Katsunuma S;Doi K;Nibu K.
为了进一步了解生长因子在嗅神经发生中的作用，我们研究了嗅球（OB）和嗅上皮（OE）再生过程中不同基因的mRNA水平。通过DNA微阵列分析研究了在OE从他巴唑给药诱导的损伤中再生过程中OB和OE中各种基因的mRNA表达水平。结果通过实时定量RT-PCR和免疫组织化学得到证实。观察期间各种基因的表达水平发生了显着变化。其中，BDNF mRNA 表达在 OE 中的前 7 天内急剧增加，然后下降。相反，OB中BDNF的mRNA表达在给药后的前7天内显着下降，然后逐渐上升。 OB中OMP mRNA水平的变化与OE和OB中OMP mRNA水平的变化完全一致。目前的结果表明，OE 中的 BDNF 有助于早期再生，而 OB 中的 BDNF 在嗅觉受体神经元 (ORN) 的再生后期发挥作用。
To further understand the roles of growth factors in the olfactory neurogenesis, we studied the mRNA levels of diverse genes in olfactory bulb (OB) and olfactory epithelium (OE) during the regeneration process of OE. mRNA expression levels of various genes in the OB and OE during the regeneration processes of OE from damage induced by methimazole administration were studied by DNA microarray analysis. The results were confirmed by quantitative real-time RT-PCR and immunohistochemistry. Expression levels of various genes dramatically changed during the observation period. Among them, mRNA expression of BDNF dramatically increased in OE during the first 7 days and then decreased. In contrast, mRNA expression of BDNF in OB significantly decreased during the first 7 days after administration and then gradually increased. The changes in the mRNA levels of OMP in OB precisely followed those of OMP in OE and OB. The present results suggest that BDNF in OE contributes to the early stage of regeneration, and BDNF in OB has its role in the late stage of regeneration of olfactory receptor neurons (ORNs).