The fine structure of connective tissue. I. The fibroblast
The fine structure of connective tissue. I. The fibroblast
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DOI：
10.1016/0014-4800(62)90040-0
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发表时间：
1962-01-01
期刊：
影响因子：
--
通讯作者：
FERNANDO, NEIL V. P.
中科院分区：
文献类型：
--
作者：
MOVAT, HENRY Z.;FERNANDO, NEIL V. P.
用电镜观察再生肌腱、肉芽组织及其它组织的超微结构。光镜下鉴定细胞为成纤维细胞，并制备电镜切片。成纤维细胞（特别是增殖中的成纤维细胞）很难充分保存[长破折号]，线粒体肿胀和破坏经常发生。电子显微镜下可见成纤维细胞是细长的梭形或星状细胞，细胞核略呈锯齿状。在休眠细胞中，细胞质逐渐变细，形成细长的突起。静息和增殖细胞都含有ergastoplasm（内质网的粗糙表面小泡），但这在增殖细胞中更丰富。ergastoplasm有时含有电子致密物质，它可能是膨胀的。细胞的外围无造质，但常含有细丝。在增殖的成纤维细胞中，总是可以看到一个大的高尔基体。细胞质中密集的嗜锇星状小体是成纤维细胞的典型特征。小颗粒或小球出现在高尔基体区域，稍大的颗粒出现在细胞质的周围。大部分颗粒类似于分泌颗粒。其他的看起来像是摄入的物质或结构，被描述为溶酶体。在高尔基体区域有一个令人费解的结构，它是一个纺锤形的身体，含有被磷钨酸强烈染色的丝状物质。|| 摘要编辑：作者
The fine structure of regenerating tendon, granulation tissue and various other tissues was studied by electron microscopy. The cells were identified as fibroblasts with the light microscope, and then ultrathin sections were prepared for electron microscopy. Fibroblasts (particularly proliferating ones) are difficult to preserve adequately[long dash]swelling and disruption of mitochondria occurring frequently. Fibroblasts as seen by electron microscopy are elongated spindle-shaped or stellate cells with a slightly indented nucleus. In the resting cell the cytoplasm may taper off into long slender processes. Both resting and proliferating cells contain ergastoplasm (rough-surfaced vesicles of the endo-plasmic reticulum), but this is more abundant in proliferating cells. The ergastoplasm sometimes contains an electron dense substance and it may be distended. The periphery of the cell is free of ergastoplasm but often contains fine filaments. A large Golgi apparatus was always seen in proliferating fibroblasts. Dense osmiophilic stellate bodies in the cytoplasm are typical of the fibroblast. Small granules or globules occur in the Golgi region, and somewhat larger ones are found in the periphery of the cytoplasm. Most of the granules resemble secretory granules. Others look like ingested material or structures described as lysosomes. A puzzling structure in the Golgi area is a spindle-shaped body containing filamentous material which stains intensely with phosphotungstic acid. || ABSTRACT AUTHORS: Authors