A differential medium for lactic acid-producing bacteria in a mixed culture
A differential medium for lactic acid-producing bacteria in a mixed culture
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DOI：
10.1111/j.1472-765x.2008.02371.x
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发表时间：
2008-06-01
影响因子：
2.4
通讯作者：
Lee, Y.
中科院分区：
文献类型：
--
作者：
Lee, H. M.;Lee, Y.
目的：采用改良的含溴酚蓝的deMan-Rogosa Sharpe琼脂（mMRS-BPB）作为培养基，对10种乳酸菌（Lactobacillus acidophilus，L. brevis，短叶湖L. bulgaricus、保加利亚湖L.加塞里湖类干酪L. [医]植物reuteri、Weissella confusa、Bifidobacterium bifidum和B.和五种市售酸奶接种在具有溴甲酚紫、mMRS和mMRS-BPB的平板计数琼脂上。每种类型的菌株在厌氧条件下比在有氧条件下在三种培养基上显示出更清楚地形成的菌落。特别是每种类型菌株在mMRS-BPB上产生具有每种物种特定特征的菌落。结论：在厌氧条件下，含溴酚蓝的改良deMan-Rogosa Sharpe琼脂适合计数和区分混合培养物中的每种LAB。本研究的意义和影响：改良型deMan-含有溴酚蓝的Rogosa Sharpe琼脂可用于从发酵食品中分离和计数每种LAB以及肠道微生物菌群。
Aims: Modified deMan-Rogosa Sharpe agar containing bromophenol blue (mMRS-BPB) was tested as a medium for counting and differentiation of each lactic acid-producing bacterium (LAB), especially in a mixed culture.Methods and Results: Type strains of 10 LAB species (Lactobacillus acidophilus, L. brevis, L. bulgaricus, L. gasseri, L. paracasei, L. plantarum, L. reuteri, Weissella confusa, Bifidobacterium bifidum and B. infantis) and five commercial yogurts were inoculated on plate count agar with bromocresol purple, mMRS, and mMRS-BPB. Each type strain showed more clearly formed colonies on the three media under anaerobic conditions than under aerobic conditions. Especially each type strain produced colonies with specific characteristics of each species on mMRS-BPB. Commercial yogurts produced the largest number of colonies with various shapes and colours on mMRS-BPB.Conclusions: Modified deMan-Rogosa Sharpe agar containing bromophenol blue under anaerobic conditions is appropriate for counting and differentiating each LAB in a mixed culture.Significance and Impact of the Study: Modified deMan-Rogosa Sharpe agar containing bromophenol blue will be useful in isolation and enumeration of each LAB from fermented foods as well as intestinal microflora.