COLLECTION, CRYOPRESERVATION AND SUBSEQUENT VIABILITY OF HEMATOPOIETIC STEM-CELLS INTENDED FOR TREATMENT OF CHRONIC GRANULOCYTIC LEUKEMIA IN BLAST-CELL TRANSFORMATION
COLLECTION, CRYOPRESERVATION AND SUBSEQUENT VIABILITY OF HEMATOPOIETIC STEM-CELLS INTENDED FOR TREATMENT OF CHRONIC GRANULOCYTIC LEUKEMIA IN BLAST-CELL TRANSFORMATION
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DOI：
10.1111/j.1365-2141.1978.tb03656.x
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发表时间：
1978-01-01
影响因子：
6.5
通讯作者：
RUUTU, T
中科院分区：
文献类型：
--
作者：
GOLDMAN, JM;THNG, KH;RUUTU, T
将从47名慢性期慢性粒细胞白血病[CGL]患者收集的外周血血沉棕黄层（BC）和骨髓（BM）细胞储存在-196 ℃。C.采用10%二甲基亚砜（DMSO）作为冷冻保护剂。由于这些细胞包括CFUc（集落形成单位）和可能的多能干细胞，它们可以作为进入母细胞转化的患者管理的一部分进行输血。每个程序收集和储存的有核细胞的平均数量对于BC收集比对于BM收获高约9倍[106 . ±. 49（SD）×109对11.9 ±。6.6. times. 109、分别。琼脂集落形成单位（CFUc）测定表明，储存的细胞可存活长达5年。由于体外研究表明，CFUc增殖不受低浓度DMSO的抑制，因此在输注前细胞重建期间可能不需要去除所有DMSO。如果自体BC细胞能够在母细胞转化中重新填充接受CGL治疗的患者的BM，则对于所有新诊断的患者，可能需要常规收集和储存BC而不是BM细胞。
Peripheral blood buffy coat (BC) and bone marrow (BM) cells, collected from 47 patients with chronic granulocytic leukemia [CGL] in the chronic phase, were stored at -196.degree. C. Dimethyl sulfoxide (DMSO) 10% was used as cryoprotective agent. As these cells included CFUc [colony-forming units] and probably pluripotential stem cells, they may be transfused as part of the management of patients who enter blast cell transformation. The mean numbers of nucleated cells collected and stored per procedure was about 9 times greater for BC collections than for BM harvests [106 .+-. 49 (SD) .times. 109 vs. 11.9 .+-. 6.6 .times. 109, respectively]. Agar CFUc assay showed that stored cells may remain viable for up to 5 yr. Since in vitro studies showed that CFUc proliferation was not inhibited by low concentrations of DMSO, the removal of all DMSO during cell reconstitution before transfusion may not be necessary. If autologous BC cells are capable of repopulating the BM of patients treated for CGL in blast cell transformation the routine collection and storage of BC rather than BM cells may be desirable for all newly diagnosed patients.