NODULES WITH ACTIVATED DEFENSE 1 is required for maintenance of rhizobial endosymbiosis in Medicago truncatula
NODULES WITH ACTIVATED DEFENSE 1 is required for maintenance of rhizobial endosymbiosis in Medicago truncatula
复制标题
具有激活防御的结节 1 是维持蒺藜苜蓿根瘤菌内共生所必需的
DOI：
10.1111/nph.14017
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发表时间：
2016
期刊：
影响因子：
9.4
通讯作者：
Zhang Zhongming
中科院分区：
文献类型：
--
作者：
Wang Chao;Yu Haixiang;Luo Li;Duan Liujian;Cai Liuyang;He Xinxing;Wen Jiangqi;Mysore Kirankumar S.;Li Guoliang;Xiao Aifang;Duanmu Deqiang;Cao Yangrong;Hong Zonglie;Zhang Zhongming
The symbiotic interaction between legume plants and rhizobia results in the formation of root nodules, in which symbiotic plant cells host and harbor thousands of nitrogen‐fixing rhizobia.Here, aMedicago truncatula nodules with activated defense 1(nad1) mutant was identified using reverse genetics methods. The mutant phenotype was characterized using cell and molecular biology approaches. An RNA‐sequencing technique was used to analyze the transcriptomic reprogramming ofnad1mutant nodules.In thenad1mutant plants, rhizobial infection and propagation in infection threads are normal, whereas rhizobia and their symbiotic plant cells become necrotic immediately after rhizobia are released from infection threads into symbiotic cells of nodules. Defense‐associated responses were detected innad1nodules.NAD1is specifically present in root nodule symbiosis plants with the exception ofMorus notabilis, and the transcript is highly induced in nodules.NAD1encodes a small uncharacterized protein with two predicted transmembrane helices and is localized at the endoplasmic reticulum.Our data demonstrate a positive role for NAD1 in the maintenance of rhizobial endosymbiosis during nodulation.
