Inhibition of HCV replicon cell growth by 2-arylbenzofuran derivatives isolated from Mori Cortex Radicis
Inhibition of HCV replicon cell growth by 2-arylbenzofuran derivatives isolated from Mori Cortex Radicis
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DOI：
10.1055/s-2007-990249
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发表时间：
2007-11-01
期刊：
影响因子：
2.7
通讯作者：
Hwang, Soon Bong
中科院分区：
文献类型：
--
作者：
Lee, Hyun Yong;Yum, Ji Hyun;Hwang, Soon Bong
草药越来越多地用于寻找安全有效的丙型肝炎病毒感染候选药物。在本研究中，我们研究了桑皮中化合物的抗hcv作用。在对173种药用植物中具有抗hcv亲和力的提取物进行筛选时，选择了桑皮的甲醇提取物。通过基于复制子细胞的检测对提取物进行抗病毒活性的分离，从乙酸乙酯可溶性部分分离出五种化合物：桑葚苷C(1)、moracin P(2)、moracin O(3)、moracin M(4)和mulberrofuran K(5)。化合物1和化合物4具有明显的抑制活性。化合物2和3在复制子细胞实验中表现出较强的抑制活性（IC50分别为35.6 μ M, 80.8 μ M），并通过抑制NS3解旋酶活性（IC50分别为42.9 μ M, 27.0 μ M）证实了这一点。
Medicinal herbs are increasingly used in the search for safe and efficient drug candidates for hepatitis C virus infection. In this study, we have investigated the anti-HCV effect of compounds from Mori Cortex Radicis. During a screening for extracts with anti-HCV affinity from medicinal plants (173 species), the methanol extract of Mori Cortex Radicis was selected. Fractionation of the extract by monitoring antiviral activity with a replicon cell-based assay resulted in the isolation of five compounds, mulberroside C (1), moracin P (2), moracin O (3), moracin M (4) and mulberrofuran K (5) from the ethyl acetate-soluble fraction. Compounds 1 similar to 4 showed significant inhibitory activities. Compounds 2 and 3 showed potent inhibitory activity (IC50 35.6 mu M, 80.8 mu M, respectively) in the replicon cell assay, which was confirmed by NS3 helicase inhibitory activity (IC50 42.9 mu M, 27.0 mu M, respectively).