Promoter regions of cysteine endopeptidase genes from legumes confer germination-specific expression in transgenic tobacco seeds
Promoter regions of cysteine endopeptidase genes from legumes confer germination-specific expression in transgenic tobacco seeds
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豆类半胱氨酸内肽酶基因的启动子区域赋予转基因烟草种子发芽特异性表达
DOI：
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发表时间：
2004
影响因子：
5.1
通讯作者：
T. Minamikawa
中科院分区：
文献类型：
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作者：
D. Yamauchi;Y. Terasaki;T. Okamoto;T. Minamikawa
半胱氨酸内肽酶（来自绿豆的SH-EP和来自菜豆的EP-C1）在种子萌发过程中起降解种子贮藏蛋白的作用。以转基因烟草为材料，分析了SH-EP基因在转基因烟草中的表达和EP-C1基因启动子活性。这两个基因的启动子在烟草种子中表现出萌发特异性激活，虽然SH-EP的翻译后加工和EP-C1基因启动子的调控区被发现在豆科植物种子和转基因烟草种子之间存在差异。
Cysteine endopeptidases, SH-EP from Vigna mungo and EP-C1 from Phaseolus vulgaris, act to degrade seed storage protein during seed germination. Using transgenic tobacco plants, expression of SH-EP and promoter activity of the EP-C1 gene were analyzed in transgenic tobacco plants. The promoters of the two genes in tobacco seeds showed germination-specific activation, although post-translational processing of SH-EP and regulatory regions of promoter of the gene for EP-C1 were found to differ between leguminous seeds and transgenic tobacco seeds.
DOI：
10.1073/pnas.83.20.7721
发表时间：
1986-10-01
影响因子：
11.1
作者：
CHAN, SJ;SANSEGUNDO, B;STEINER, DF
通讯作者：
STEINER, DF