VARIATIONS IN ATP-SENSITIVE K+ CHANNEL ACTIVITY PROVIDE EVIDENCE FOR INHERENT METABOLIC OSCILLATIONS IN PANCREATIC BETA-CELLS
VARIATIONS IN ATP-SENSITIVE K+ CHANNEL ACTIVITY PROVIDE EVIDENCE FOR INHERENT METABOLIC OSCILLATIONS IN PANCREATIC BETA-CELLS
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DOI：
10.1006/bbrc.1994.2746
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发表时间：
1994-11-30
影响因子：
3.1
通讯作者：
HELLMAN, B
中科院分区：
文献类型：
--
作者：
DRYSELIUS, S;LUND, PE;HELLMAN, B
膜片钳技术的细胞附着配置用于研究从小鼠和人分离的胰腺β细胞中ATP敏感性K+通道活性的缓慢变化。在0或3 mM葡萄糖中，通道开放的时间分数显示频率在0.25-0.40 /min范围内的振荡。这种现象是β细胞ATP产生的内在波动的有力论据。因此，代谢的变化可能是细胞质Ca 2+以相等频率的特征性大振幅振荡的主要决定因素。(C)1994年出版社出版。
The cell-attached configuration of the patch clamp technique was used for studying slow variations in the activity of the ATP-sensitive K+ channels in pancreatic beta-cells isolated from mouse and man. In 0 or 3 mM glucose, the fraction of time the channels were open exhibited oscillations with frequencies in the 0.25-0.40 /min range. This phenomenon is a strong argument for inherent fluctuations in the ATP production of the beta-cells. variations in metabolism may thus be a major determinant for the characteristic large amplitude oscillations of cytoplasmic Ca2+ with equivalent frequency. (C) 1994 Academic Press, Inc.