Basonuclin, a zinc finger protein of keratinocytes and reproductive germ cells, binds to the rRNA gene promoter
Basonuclin, a zinc finger protein of keratinocytes and reproductive germ cells, binds to the rRNA gene promoter
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DOI：
10.1073/pnas.96.17.9628
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发表时间：
1999-08-17
影响因子：
11.1
通讯作者：
Green, H
中科院分区：
文献类型：
--
作者：
Iuchi, S;Green, H
Basonuclin是一种含有三对C2H2锌指的蛋白质。这种蛋白质存在于复层鳞状上皮（如表皮）的基底（生殖）细胞层以及睾丸和卵巢的生殖细胞中。我们在这里表明，人类蛋白质具有特定的亲和力的rRNA基因的启动子的一段，Basonuclin相互作用的启动子的两个独立的部分，每个具有二分体对称性。Basonuclin的上游部分与rDNA的转录因子UBF 1结合，而下游部分与UBF 1结合，Basonuclin可能是rDNA转录的细胞类型特异性调控蛋白。
Basonuclin is a protein containing three pairs of C2H2 zinc fingers. The protein has been found in the basal (germinal) cell layer of stratified squamous epithelia, such as the epidermis, and in germ cells of the testis and ovary. We show here that the human protein has specific affinity for a segment of the promoter of the gene for rRNA, Basonuclin interacts with two separate parts of the promoter, each possessing dyad symmetry. The upstream part, but not the downstream part, is known to bind UBF1, a transcription factor for rDNA, Basonuclin is likely to be a cell-type-specific regulatory protein for rDNA transcription.