Achieving stable anammox process and revealing shift of bacteria during the start‐up in landfill leachate treatment
Achieving stable anammox process and revealing shift of bacteria during the start‐up in landfill leachate treatment
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DOI：
10.1002/wer.10841
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发表时间：
2023-01
影响因子：
3.1
通讯作者：
Chun-Min Jia;Lina Wu;Ke Yu;Jincheng Hu;Jia Qi;Anteng Luo
中科院分区：
文献类型：
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作者：
Chun-Min Jia;Lina Wu;Ke Yu;Jincheng Hu;Jia Qi;Anteng Luo
部分硝化-厌氧氨氧化（PN/A）是一种高效节能的垃圾渗滤液脱氮技术。许多研究报告成功地实施了PN/A工艺，并在实验室条件下稳定运行。PN/A工程应用的主要挑战之一是启动所需的种子污泥质量。采用序批式活性污泥法（SBR）处理垃圾渗滤液，考察了PN/A值。经过70天的运行，该系统成功实现了一级PN/A工艺，并保持了稳定的NH 4 +$$ \left（{NH}_4^{+}\right）$$去除率为97.65% ± 1%，其中NH 4 +$$ {NH}_4^{+} $$和硝酸盐（NO3-$$ {NO}_3^{-} $$）的流出物小于4 ± 1.5 mg L−1和10 mg L−1。Ca. Kuenenia和Ca.典型的厌氧氨氧化菌（AnAOB）Brocadia分别从0.08%增加到3.99%（70天）和0.01%增加到0.45%。氨氧化细菌（AOB）亚硝化单胞菌和亚硝化螺旋菌的相对丰度分别从0.9%增加到2.89%和0.007%增加到0.1%（70天）。AnAOB和AOB都是系统的重要生态位。
Partial nitrification‐anammox (PN/A) is an energy‐efficient technology for nitrogen removal in landfill leachate treatment. Numerous studies have reported successful implementation of the PN/A process and its stable operation under laboratory conditions. One of the primary challenges in PN/A engineering applications is the mass of the seed sludge required for start‐up. This study examined the PN/A using a sequence batch reactor (SBR) inoculating a common mixture to treat landfill leachate. After 70 days of operation, the system successfully realized a one‐stage PN/A process and maintained a stable ammonium NH4+$$ \left({NH}_4^{+}\right) $$ removal efficiency of 97.65% ± 1%, where the effluent of NH4+$$ {NH}_4^{+} $$ and nitrate ( NO3−$$ {NO}_3^{-} $$ ) were less than 4 ± 1.5 mg L−1 and 10 mg L−1. In addition, the relative abundances of Ca. Kuenenia and Ca. Brocadia, which are typical anaerobic ammonia‐oxidizing bacteria (AnAOB), increased from 0.08% to 3.99% (70 days) and 0.01% to 0.45%, respectively. The relative abundances of ammonia‐oxidizing bacteria (AOB) Nitrosomonas and Nitrosospira increased from 0.9% to 2.89% and 0.007% to 0.1% (70 days), respectively. Both AnAOB and AOB are important niches of the system.