Multiple Wnt signaling pathways converge to orient the mitotic spindle in early C. elegans embryos
Multiple Wnt signaling pathways converge to orient the mitotic spindle in early C. elegans embryos
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DOI：
10.1016/j.devcel.2004.10.008
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发表时间：
2004-12-01
影响因子：
11.8
通讯作者：
Hardin, J
中科院分区：
文献类型：
--
作者：
Walston, T;Tuskey, C;Hardin, J
细胞如何在分裂过程中整合多个极化信号的输入，目前尚不清楚。我们证明了两种不同的秀丽隐杆线虫Wnt通路通过不同地调节其复制中心体来促进ABar卵裂球的极化。与C卵裂球的接触通过非转录的Wnt纺锤体对齐途径定向ABar纺锤体，而Wnt/ β -连环蛋白途径控制ABar纺锤体旋转的时间。三种线虫disheveled同源物以不同的方式参与这些过程，表明它们之间可能存在功能差异。我们还发现CKI （KIN-19）不仅在Wnt/ β -catenin通路中起作用，而且在Wnt纺锤体取向通路中也起作用。基于这些发现，我们建立了细胞间相互作用和不同Wnt信号通路的协调模型，以确保在发育调节的细胞分裂事件中纺锤体旋转的稳健时间和方向。
How cells integrate the input of multiple polarizing signals during division is poorly understood. We demonstrate that two distinct Caenorhabditis elegans Wnt pathways contribute to the polarization of the ABar blastomere by differentially regulating its duplicated centrosomes. Contact with the C blastomere orients the ABar spindle through a nontranscriptional Wnt spindle alignment pathway, while a Wnt/beta-catenin pathway controls the timing of ABar spindle rotation. The three C. elegans Dishevelled homologs contribute to these processes in different ways, suggesting that functional distinctions may exist among them. We also find that CKI (KIN-19) plays a role not only in the Wnt/beta-catenin pathway, but also in the Wnt spindle orientation pathway as well. Based on these findings, we establish a model for the coordination of cell-cell interactions and distinct Wnt signaling pathways that ensures the robust timing and orientation of spindle rotation during a developmentally regulated cell division event.