The mRNA abundance of pepc2 gene is negatively correlated with oil content in Chlamydomonas reinhardtii
The mRNA abundance of pepc2 gene is negatively correlated with oil content in Chlamydomonas reinhardtii
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莱茵衣藻pepc2基因mRNA丰度与油脂含量呈负相关
DOI：
10.1016/j.biombioe.2011.01.005
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发表时间：
2011-05-01
影响因子：
6
通讯作者：
Fei, Xiaowen
中科院分区：
文献类型：
--
作者：
Deng, Xiaodong;Li, Yajun;Fei, Xiaowen
许多微藻积累油脂，特别是在胁迫条件下，因此作为生产生物柴油的潜在原料越来越受到关注。然而，很少有信息是可用于控制微藻中石油积累的分子机制。在这项研究中，我们调查了石油在氮（N）剥夺模式绿色藻类莱茵衣藻积累。结果表明，缺氮后，油分含量显著增加。莱茵衣藻（reinhardtii），其伴随磷酸烯醇丙酮酸羧化酶2（PEPC 2）的转录水平的急剧降低（63-91%），所述磷酸烯醇丙酮酸羧化酶2是将磷酸烯醇丙酮酸（PEP）转化为草酰乙酸（OAA）的酶，所述草酰乙酸与乙酰辅酶A缀合以产生柠檬酸。此外，通过RNAi沉默pepc 2基因在C. reinhardtii导致油含量增加14-28%。这些结果表明，pepc 2 mRNA丰度与油分含量呈负相关。莱因哈德氏菌(C)2011爱思唯尔有限公司保留所有权利。
Many microalgae accumulate oils, especially under stress conditions, thus have attracted more and more attention as a potential feedstock for biodiesel production. However, little information is available for molecular mechanisms that control oil accumulation in microalgae. In this study, we investigated the accumulation of oils during nitrogen (N) deprivation in the model green algae Chlamydomonas reinhardtii. Our results indicated that oil content significantly increased after N deficiency in C. reinhardtii, which was accompanied by a drastic decrease (63-91%) in the transcript level of phosphoenolpyruvate carboxylase 2 (PEPC2), an enzyme that converts phosphoenolpyruvate (PEP) to oxaloacetate (OAA), which conjugates with acetyl-CoA to generate citric acid. Furthermore, down regulation of pepc2 gene by RNAi silencing in C. reinhardtii led to 14-28% increase in oil content. These results revealed a negative correlation between pepc2 mRNA abundance and oil content in C. reinhardtii. (C) 2011 Elsevier Ltd. All rights reserved.