ERRATUM
ERRATUM
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DOI：
10.1002/stem.3177
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发表时间：
2020-04
期刊：
影响因子：
5.2
通讯作者：
V. Brochard;L. Jouneau;A. Bonnet-Garnier;H. Jammes;L. Vallier;M. I.Gabrielle;Brons;A. Jouneau
中科院分区：
文献类型：
--
作者：
V. Brochard;L. Jouneau;A. Bonnet-Garnier;H. Jammes;L. Vallier;M. I.Gabrielle;Brons;A. Jouneau
2010年发表的上述文章检查了来自受精胚胎（FT-EpiSC）或体细胞核移植胚胎（NT-EpiSC）的上胚层干细胞（EpiSC）的衍生过程和转录组。从卵丘细胞注射到从B6 D2 F1小鼠收集的卵母细胞中获得核移植胚胎。虽然衍生过程本身是非常相似的，无论细胞的起源，通过微阵列的转录组的比较显示了少量（25）的差异表达的基因之间的2个NT线，与3个FT线。第三条线（称为NT-1）与FT相似。最近，对不同的EpiSC系（包括之前表征的那些）进行了RNA测序，目的是研究多能细胞中的等位基因使用和印记[Dirks et al. Epigenetics & Chromatin 2019;12：14]。由于它们的杂交基因型（B6 D2 F1），等位基因特异性定位是可能的。这种深入分析意外地揭示了两种转录异常的EpiSC（先前标记为NT-2和NT-LE）的重组基因型结构仅与单性生殖起源相容。早期研究中使用的第三个品系NT-1根据其基因型显然是NT起源的。DOI：10.1002/stem.3177
The above-referenced article, published in 2010, examined the derivation process and the transcriptome of epiblast stem cells (EpiSCs) derived from either fertilized embryos (FT-EpiSCs) or somatic nuclear-transferred embryos (NT-EpiSCs). Nuclear-transferred embryos were obtained from cumulus cells injected into oocytes collected from B6D2F1 mice. Although the derivation process itself was very similar regardless of the origin of the cells, the comparison of the transcriptome by microarray did show a small number (25) of differentially expressed genes between 2 out of 3 NT lines, compared with 3 FT lines. The third line (called NT-1) was similar to FT. More recently, RNA sequencing of different EpiSC lines, including those previously characterized, was performed with the aim of looking at the allelic usage and imprinting in pluripotent cells [Dirks et al. Epigenetics & Chromatin 2019;12:14]. Allele-specific mapping was possible thanks to their hybrid genotype (B6D2F1). This in-depth analysis unexpectedly revealed that the recombined genotype structure of the two transcriptionally deviant EpiSCs (previously labeled as NT-2 and NT-LE) was only compatible with a parthenote origin. The third line used in the earlier study, NT-1, was clearly of NT-origin, according to its genotype. DOI: 10.1002/stem.3177