Effects of Vasoactive Intestinal Polypeptide and Forskolin on mRNA Expression of Prolactin and Prolactin Regulatory Element-Binding Protein in the Anterior Pituitary Gland of Chicken Embryo and Laying Hens
Effects of Vasoactive Intestinal Polypeptide and Forskolin on mRNA Expression of Prolactin and Prolactin Regulatory Element-Binding Protein in the Anterior Pituitary Gland of Chicken Embryo and Laying Hens
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DOI：
10.2141/jpsa.0160039
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发表时间：
2016-10-01
影响因子：
1.5
通讯作者：
Zadworny, David
中科院分区：
文献类型：
--
作者：
Kansaku, Norio;Tobari, Yasuko;Zadworny, David
血管活性肠肽（VIP）处理诱导鸡垂体前叶泌乳素（PRL） mRNA表达。PRL启动子的VIP响应元件（VRE）在多种鸟类中得到了鉴定。然而，与VRE结合的转录因子尚未被确定。泌乳素调控元件结合蛋白（Prolactin regulatory element-binding protein， PREB）基因作为候选转录因子与VRE结合。在胚胎发生过程中，PRL和PREB mRNA水平升高。然而，在胚胎发生过程中，VIP是否影响PRL和PREB mRNA的水平尚不清楚。观察VIP和forskolin对胚胎垂体前腺PRL和PREB mRNA表达的影响。通过对蛋鸡进行VIP处理，研究VIP与PREB mRNA表达的关系。在胚胎生长的第14天，VIP处理对PRL和PREB的mRNA水平没有影响，而福斯克林处理导致PRL和PREB mRNA水平升高。第20天，VIP和forskolin均诱导PRL和PREB mRNA水平升高。VIP显著提高了白来角鼠和名古屋鼠垂体前叶PRL和PREB mRNA的表达水平。由此可见，VIP对鸡垂体前叶PRL和PREB mRNA表达水平的影响可能反过来影响垂体前叶PRL和PREB mRNA表达水平。
Vasoactive intestinal peptide (VIP) treatment induced mRNA expression of Prolactin (PRL) in the chicken anterior pituitary gland. VIP responsive element (VRE) of the PRL promoter was identified in the various bird species. However, transcription factor, which binds to VRE, has not yet been identified. Prolactin regulatory element-binding protein (PREB) gene cloned as a candidate transcription factor binds to VRE. Increases of mRNA levels of PRL and PREB during embryogenesis were identified. However, whether VIP affects levels of PRL and PREB mRNA during embryogenesis remains unknown. The effects of VIP and forskolin on mRNA expression of PRL and PREB in the embryonic anterior pituitary gland were assessed. Furthermore, administration of VIP to laying hens was conducted to examine the relationship between VIP and PREB mRNA expression. At day 14 of the embryonic growth stage, VIP treatment did not affect mRNA levels of either PRL or PREB, whereas forskolin treatment induced the increase of these mRNA levels. At day 20, both VIP and forskolin induced an increase of PRL and PREB mRNA levels. The administration of VIP significantly increased mRNA levels of PRL and PREB in the anterior pituitary gland of White Leghorn and Nagoya. These results indicate that the effects of VIP on PRL and PREB mRNA expression levels of VIP receptor may in turn affect PRL and PREB mRNA levels in the chicken anterior pituitary gland.