Prostromelysin and procollagenase genes are differentially up-regulated in chondrocytes from the knees of rabbits with experimental osteoarthritis.
Prostromelysin and procollagenase genes are differentially up-regulated in chondrocytes from the knees of rabbits with experimental osteoarthritis.
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DOI：
10.1002/art.1780370813
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发表时间：
1994-12
影响因子：
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通讯作者：
F. Mehraban;S. Kuo;H. Riera;C. Chang;R. Moskowitz
中科院分区：
文献类型：
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作者：
F. Mehraban;S. Kuo;H. Riera;C. Chang;R. Moskowitz
目的检测实验性骨关节炎（OA）兔软骨中基质金属蛋白酶（MMP）基因pro-MMP 1和pro-MMP 3的相对表达，并评价软骨细胞在此过程中的作用。方法采用家兔部分内侧髁切除术建立OA模型。在4周或8周时处死兔，从软骨中制备总细胞RNA，并用pro-MMP 32 P标记的互补DNA通过北方印迹进行探测。单层软骨细胞用于评估软骨细胞因子的MMP诱导活性。结果实验性OA软骨中MMP前体mRNA表达上调，MMP前体3 mRNA表达高于MMP前体1。OA衍生的软骨细胞的条件培养基上调正常软骨细胞中的pro-MMP mRNA。结论MMP基因在OA模型中表达上调，可能参与了OA软骨的降解。软骨细胞通过自分泌途径上调MMP基因。
OBJECTIVE To determine the relative expressions of matrix metalloprotease (MMP) genes pro-MMP1 and pro-MMP3 in the cartilage of rabbits with experimentally induced osteoarthritis (OA), and to assess the role of the chondrocyte in this process. METHODS OA was induced in rabbits after partial medial meniscectomy. Rabbits were killed at 4 weeks or 8 weeks, and total cellular RNA was prepared from cartilage and probed by Northern blotting with pro-MMP 32P-labeled complementary DNA. Monolayer chondrocytes were used to assess MMP-inducing activity of chondrocyte factor(s). RESULTS Pro-MMP messenger RNAs (mRNAs) were up-regulated in experimental OA cartilage; pro-MMP3 mRNA expression exceeded that of pro-MMP1. Conditioned medium from OA-derived chondrocytes up-regulated pro-MMP mRNAs in normal chondrocytes. CONCLUSION Up-regulation of MMP genes in this OA model may contribute to cartilage degradation. Chondrocytes up-regulate MMP genes via an autocrine pathway.