Molecular cloning and functional characterization of the apple sucrose transporter gene MdSUT2
Molecular cloning and functional characterization of the apple sucrose transporter gene MdSUT2
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DOI：
10.1016/j.plaphy.2016.10.026
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发表时间：
2016-12-01
影响因子：
6.5
通讯作者：
Hao, Yu-Jin
中科院分区：
文献类型：
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作者：
Ma, Qi-Jun;Sun, Mei-Hong;Hao, Yu-Jin
蔗糖不仅是主要的光合产物，而且是重要经济植物物种韧皮部转运的主要成分。蔗糖转运蛋白或载体（SUT 或 SUC5）充当蔗糖/H+ 同向转运蛋白，在确定蔗糖在整个植物中的细胞间分布方面发挥着至关重要的作用。然而，这些基因是否参与对非生物胁迫和其他生物过程的反应尚不清楚。在这里，我们报道苹果中的 MdSUT2 是拟南芥液泡蔗糖转运蛋白 AtSUT2 的同源物。拟南芥中 MdSUT2 的异位表达降低了发芽和播种阶段的蔗糖敏感性，并增加了蔗糖转运活性。此外，我们的结果表明，MdSUT2 通过加速拟南芥的营养生长和促进早期开花来影响植物生长。 MdSUT2 的过表达显着提高了苹果愈伤组织和拟南芥中对 NaC1、ABA 和甘露醇等非生物胁迫的耐受性。总之，这些发现提供了苹果蔗糖转运蛋白 MdSUT2 参与非生物胁迫抵抗和植物生长发育调节的证据。 (C) 2016 Elsevier Masson SAS。版权所有。
Sucrose is not only the primary photosynthetic product but also the major component translocated in the phloem of economically important plant species. Sucrose transporters or carriers (SUTs or SUC5), function as sucrose/H+ symporters and play a crucial role in determining the cell-to-cell distribution of sucrose throughout the entire plant. However, whether such genes are involved in responses to abiotic stress and other biological processes is largely unknown. Here, we report that MdSUT2 in apple is a homolog of the Arabidopsis vacuolar sucrose transporter AtSUT2. Ectopic expression of MdSUT2 in Arabidopsis decreased sucrose sensitivity in germination and seeding stage and increased sucrose transport activity. In addition, our results showed that MdSUT2 impacted on plant growth by accelerating vegetative growth and promoting early flowering in Arabidopsis. Overexpression of MdSUT2 significantly improved abiotic stress tolerance including NaC1, ABA, and mannitol in apple calli and Arabidopsis. Together, these findings provide evidence that the apple sucrose transporter MdSUT2 is involved in abiotic stress resistance and the regulation of plant growth and development. (C) 2016 Elsevier Masson SAS. All rights reserved.