Cerebellar modules in the olivo-cortico-nuclear loop demarcated by pcdh10 expression in the adult mouse
Cerebellar modules in the olivo-cortico-nuclear loop demarcated by pcdh10 expression in the adult mouse
复制标题
DOI：
10.1002/cne.24499
复制
发表时间：
2018-10-15
影响因子：
2.5
通讯作者：
Sugihara, Izumi
中科院分区：
文献类型：
--
作者：
Sarpong, Gideon A.;Vibulyaseck, Suteera;Sugihara, Izumi
小脑皮质、小脑核团和下橄榄核的相应隔室之间的地形连接形成了平行的模块，这对小脑的功能是必不可少的。与醛缩酶C(ALDOC)或Zebrin II等分子标记物标记的条纹状皮质区划相比，小脑核和下橄榄核的对应组织还不是很清楚。我们重点研究了编码细胞黏附分子原钙粘附素10(Pcdh10)基因在成年小鼠体内的表达模式。在大脑皮层，pcdh10在小叶VI-VIIa+//2+表达，在第I小叶及邻近小叶表达c+、d+、4b+、5a+，在小叶III至小叶旁分布4+//5+。在小脑核团中，PCDH10在内侧核的尾侧部和后间插核的外侧区有较强的表达，投射到延髓和红核的投射较少，投射到其他中脑、中脑和间脑区域较少。在下橄榄，PCDH10在内侧副橄榄的吻部和内侧腹尾部有较强的表达，并与中脑区联系，而不是脊髓。橄榄小脑和皮质核轴突标记证实，三个皮质pcdh10阳性区域在地形图上与核和橄榄pcdh10阳性区域相连，表明它们与橄榄-皮质-核环中的模块化结构相吻合。我们推测，这些模块中的一些通过它们的传入和传出联系参与了各种非躯体感觉运动任务。
Topographic connection between corresponding compartments of the cerebellar cortex, cerebellar nuclei, and inferior olive form parallel modules, which are essential for the cerebellar function. Compared to the striped cortical compartmentalization which are labeled by molecular markers, such as aldolase C (Aldoc) or zebrin II, the presumed corresponding organization of the cerebellar nuclei and inferior olivary nucleus has not been much clarified. We focused on the expression pattern of pcdh10 gene coding cell adhesion molecule protocadherin 10 (Pcdh10) in adult mice. In the cortex, pcdh10 was strongly expressed in (a) Aldoc-positive vermal stripes a+//2+ in lobules VI-VII, (b) paravermal narrow stripes c+, d+, 4b+, 5a+ in crus I and neighboring lobules, and (c) paravermal stripes 4+//5+ across all lobules from lobule III to paraflocculus. In the cerebellar nuclei, pcdh10 was expressed strongly in the caudal part of the medial nucleus and the lateral part of the posterior interposed nucleus which project less to the medulla or to the red nucleus than to other metencephalic, mesencephalic, and diencephalic areas. In the inferior olive, pcdh10 was expressed strongly in the rostral and medioventrocaudal parts of the medial accessory olive which has connection with the mesencephalic areas rather than the spinal cord. Olivocerebellar and corticonuclear axonal labeling confirmed that the three cortical pcdh10-positive areas were topographically connected to the nuclear and olivary pcdh10-positive areas, demonstrating their coincidence with modular structures in the olivo-cortico-nuclear loop. We speculate that some of these modules are functionally involved in various nonsomatosensorimotor tasks via their afferent and efferent connections.