Genetic population structure of Liza haematocheilus in north-western Pacific detected by amplified fragment length polymorphism markers
Genetic population structure of Liza haematocheilus in north-western Pacific detected by amplified fragment length polymorphism markers
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DOI：
10.1017/s0025315412000872
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发表时间：
2012-08
影响因子：
1.2
通讯作者：
Zhiqiang Han;Gang Han;T. Gao;Zhi-Yong Wang;Bo-nian Shui
中科院分区：
文献类型：
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作者：
Zhiqiang Han;Gang Han;T. Gao;Zhi-Yong Wang;Bo-nian Shui
已经描述了几个不同的共同血缘关系的线粒体DNA谱系，这种高度的谱系间差异提出了关于西北太平洋红鱼谱系分类地位的问题。本研究采用扩增片段长度多态性技术，对西北太平洋不同mtDNA谱系的遗传结构进行了分析，并对其独立性水平进行了评估。用4对引物组合对8个群体的91个个体共扩增出186条带，多态率为91.74%。基于Nei遗传距离的算术平均树未加权对组法揭示了两个聚类群(北枝和南枝)。分子方差分析和成对FST支持西北太平洋红鳍金线虫南北种群的分离。核群和线粒体谱系之间的不一致表明，这三个不同的谱系并不代表神秘的物种，同一样本中存在不同的线粒体谱系是长期隔离后二次接触的结果。更新世的分离和物种的生物学特性可能是导致红毛链霉菌遗传分化的原因。
Several divergent sympatry mtDNA lineages have been described in redlip mullet Liza haematocheilus, and this high inter-lineage divergence raises questions about the taxonomic status of L. haematocheilus lineages in the north-western Pacific. In this study, the amplified fragment length polymorphism technique was employed to examine genetic structure of L. haematocheilus and estimate the level of independence of the different mtDNA lineages in the north-western Pacific. A total of 186 bands were amplified from 91 individuals among 8 populations by 4 primer combinations and the percentage of polymorphic bands was 91.74%. The Unweighted Pair Group Method with Arithmetic Mean tree based on Nei genetic distance revealed two clusters (North Clade and South Clade). Molecular variance analysis and pairwise FST supported the separation of north and south populations of L. haematocheilus in the north-western Pacific. The incongruence between nuclear groups and mitochondrial lineages suggests the three distinct lineages do not represent cryptic species and the presence of divergent mitochondrial lineages in the same sample is a result of secondary contact after an extended period of isolation. The Pleistocene isolation and biological characteristics of species may be responsible for the genetic differentiation of L. haematocheilus.