Cyclodextrin supramolecular complex as a water-soluble ratiometric sensor for ferric ion sensing.
Cyclodextrin supramolecular complex as a water-soluble ratiometric sensor for ferric ion sensing.
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DOI：
10.1021/la9033244
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发表时间：
2010-03
期刊：
影响因子：
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通讯作者：
Meiyun Xu;Shuizhu Wu;Fang Zeng;Changmin Yu
中科院分区：
文献类型：
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作者：
Meiyun Xu;Shuizhu Wu;Fang Zeng;Changmin Yu
对人类健康和环境保护的高度关注刺激了对过渡金属离子的潜在影响及其毒性效应的积极研究，因此非常需要设计成本有效、快速、简便并且适用于环境和生物环境的过渡金属离子检测方法。在这项研究中，我们展示了一种替代策略，通过形成超分子β-环糊精/染料复合物，构建一个水溶性FRET为基础的比率传感器的三价铁离子检测。该水溶性FRET系统由丹磺酰基连接的β-环糊精（β-CD-DNS）和螺内酰胺罗丹明连接的金刚烷（AD-SRhB）组成。丹磺酰基部分作为供体，螺内酰胺-罗丹明B衍生物（SRh B）被选为一个敏感的，选择性的化学传感器的Fe（III）离子和一个非常有效的开环反应诱导的Fe（III）产生的长波长的罗丹明B荧光团，可以作为能量受体。此外，金刚烷基（AD）基团，这是已知的能力，形成稳定的主机-客体包合物与β-CD衍生物，共价连接到螺内酰胺罗丹明，因此金刚烷基部分的离子识别元件可以锚定在CD腔。通过这种方式，供体-受体分离可以保持在临界福斯特距离内;因此，可以发生从供体（丹磺酰基）到受体（罗丹明衍生物/Fe（III）络合物）的能量转移，从而可以实现水性介质中Fe（III）的比率检测。这种基于FRET的超分子传感器可以通过使用供体部分和受体部分的包合过程容易地形成，因此这种策略可以提供一种稳健的方法，用于简单地通过组装专门预先设计的供体连接的CD和受体连接的金刚烷来构建广泛的基于FRET的水溶性传感系统。
Heightened concern for human health and environmental protection has stimulated active research on the potential impact of transition-metal ions and their toxic effects, thus it is very demanding to design transition-metal ion detection methods that are cost-effective, rapid, facile, and applicable to the environmental and biological milieus. In this study, we demonstrated an alternative strategy for constructing a water-soluble FRET-based ratiometric sensor for ferric ion detection by forming a supramolecular beta-cyclodextrin/dye complex. This water-soluble FRET system consists of a dansyl-linked beta-cyclodextrin (beta-CD-DNS) and a spirolactam rhodamine-linked adamantane (AD-SRhB). The dansyl moiety serves as the donor, and the spirolactam-rhodamine B derivative (SRhB) was chosen as a sensitive, selective chemosensor for Fe(III) ions and a very efficient ring-opening reaction induced by Fe(III) generates the long-wavelength rhodamine B fluorophore that can act as the energy acceptor. Moreover, the adamantyl (AD) group, which is known for its capability to form stable host-guest inclusion complexes with beta-CD derivatives, was covalently linked to the spirolactam rhodamine, thus the adamantyl moiety of the ion-recognition element can be anchored inside the CD cavity. In this way, the donor-acceptor separation can be kept within the critical Forster distance; accordingly, energy transfer can take place from the donor (dansyl) to the acceptor (rhodamine derivative/Fe(III) complex), and thus ratiometric detection for Fe(III) in an aqueous medium can be fulfilled. This FRET-based supramolecular sensor can be readily formed via an inclusion process using the donor part and the acceptor part, hence this strategy could afford a robust approach for constructing a wide range of FRET-based water-soluble sensing systems simply by assembling a specifically predesigned donor-linked CD and acceptor-linked adamantane.