The autophagy gene BbATG5, involved in the formation of the autophagosome, contributes to cell differentiation and growth but is dispensable for pathogenesis in the entomopathogenic fungus Beauveria bassiana
The autophagy gene BbATG5, involved in the formation of the autophagosome, contributes to cell differentiation and growth but is dispensable for pathogenesis in the entomopathogenic fungus Beauveria bassiana
复制标题
自噬基因 BbATG5 参与自噬体的形成，有助于细胞分化和生长，但对于昆虫病原真菌白僵菌的发病机制来说是可有可无的
DOI：
10.1099/mic.0.062646-0
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发表时间：
2013-02-01
期刊：
影响因子：
2.8
通讯作者：
Ying, Sheng-Hua
中科院分区：
文献类型：
--
作者：
Zhang, Lei;Wang, Jie;Ying, Sheng-Hua
自噬是一个高度保守的过程，代表了细胞成分的主要真核降解途径。自噬介导的细胞材料回收有助于细胞分化，组织重塑和正常发育。在真菌中，正常生长和细胞分化需要自噬。 Bassiana及其无脊椎动物靶标的昆虫致病真菌代表了一个独特的模型系统，可检查宿主病原体相互作用。 ATG5基因是涉及自噬体形成的17个基因之一，并且鉴定出B. bassiana同源物（BBATG5）。自噬在Bassiana生长和毒力中的作用是通过构建靶向基因敲除BBATG5的。与野生型父母相比，突变菌株对营养限制的敏感性增加，发芽和生长降低。分生物严重损害，并从三角洲BBATG5菌株中得出的分生孢子改变了形态学。细胞分化为胚孢子也大大减少了。尽管生长显着和发育缺陷，但使用东方叶虫的昆虫生物测定，Spodoptera Litura，表明Delta BBATG5菌株的毒力降低（相当于40％）。 Delta Bbatg5菌株的表型缺陷可以通过引入完整的BBATG5副本来恢复。这些数据表明，与几种植物和动物的致病真菌（在感染中需要ATG5）不同，在B. bassiana中，它对于发病机理来说是可分配的。
Autophagy is a highly conserved process, representing the major eukaryotic degradative pathway of cellular components. Autophagy-mediated recycling of cellular materials contributes to cell differentiation, tissue remodelling and proper development. In fungi, autophagy is required for normal growth and cell differentiation. The entomopathogenic fungus Beauveria bassiana and its invertebrate targets represent a unique model system with which to examine host pathogen interactions. The ATG5 gene is one of 17 involved in autophagosome formation, and the B. bassiana homologue (BbATG5) was identified. The role of autophagy in B. bassiana growth and virulence was investigated via construction of a targeted gene knockout of BbATG5. The mutant strain displayed increased sensitivity to nutrient limitation, with decreased germination and growth as compared with the wild-type parent. Conidiation was severely compromised and conidia derived from the Delta BbATG5 strain were altered in morphology. Cell differentiation into blastospores was also greatly reduced. Despite the significant growth and developmental defects, insect bioassays using the oriental leafworm moth, Spodoptera litura, indicated a modest (similar to 40%) decrease in virulence in the Delta BbATG5 strain. The phenotypic defects of the Delta BbATG5 strain could be restored by introduction of an intact copy of BbATG5. These data suggest that unlike several plant and animal pathogenic fungi, where ATG5 is required for infection, in B. bassiana it is dispensable for pathogenesis.