Structural basis of Qng1-mediated salvage of the micronutrient queuine from queuosine-5'-monophosphate as the biological substrate.
Structural basis of Qng1-mediated salvage of the micronutrient queuine from queuosine-5'-monophosphate as the biological substrate.
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DOI：
10.1093/nar/gkac1231
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发表时间：
2023-01-25
影响因子：
14.9
通讯作者：
Swairjo, Manal A.
中科院分区：
文献类型：
--
作者：
Hung, Shr-Hau;Elliott, Gregory, I;Ramkumar, Thakku R.;Burtnyak, Lyubomyr;McGrenaghan, Callum J.;Alkuzweny, Sana;Quaiyum, Samia;Iwata-Reuyl, Dirk;Pan, Xiaobei;Green, Brian D.;Kelly, Vincent P.;De Crecy-Lagard, Valerie;Swairjo, Manal A.
Eukaryotic life benefits from—and ofttimes critically relies upon—the de novo biosynthesis and supply of vitamins and micronutrients from bacteria. The micronutrient queuosine (Q), derived from diet and/or the gut microbiome, is used as a source of the nucleobase queuine, which once incorporated into the anticodon of tRNA contributes to translational efficiency and accuracy. Here, we report high-resolution, substrate-bound crystal structures of the Sphaerobacter thermophilus queuine salvage protein Qng1 (formerly DUF2419) and of its human ortholog QNG1 (C9orf64), which together with biochemical and genetic evidence demonstrate its function as the hydrolase releasing queuine from queuosine-5′-monophosphate as the biological substrate. We also show that QNG1 is highly expressed in the liver, with implications for Q salvage and recycling. The essential role of this family of hydrolases in supplying queuine in eukaryotes places it at the nexus of numerous (patho)physiological processes associated with queuine deficiency, including altered metabolism, proliferation, differentiation and cancer progression. Queuosine is a microbiome-derived 7-deazaguanosine tRNA nucleoside important for the gut-brain axis. We report the structure and function of QNG1 as a queuosine-5′-monophosphate hydrolase essential for salvage of the nucleobase queuine from exogenous queuosine, following its phosphorylation by a cellular kinase.
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影响因子：
14.9
作者：
Hashimoto H;Zhang X;Cheng X
通讯作者：
Cheng X
影响因子：
5.7
作者：
Dalhus, Bjorn;Forsbring, Monika;Bjoras, Magnar
通讯作者：
Bjoras, Magnar
DOI：
10.1016/j.bbapap.2012.10.005
发表时间：
2013-01
影响因子：
3.2
作者：
Brooks, Sonja C.;Adhikary, Suraj;Rubinson, Emily H.;Eichman, Brandt F.
通讯作者：
Eichman, Brandt F.
DOI：
10.1107/s0907444909052925
发表时间：
2010-02
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Adams PD;Afonine PV;Bunkóczi G;Chen VB;Davis IW;Echols N;Headd JJ;Hung LW;Kapral GJ;Grosse-Kunstleve RW;McCoy AJ;Moriarty NW;Oeffner R;Read RJ;Richardson DC;Richardson JS;Terwilliger TC;Zwart PH
通讯作者：
Zwart PH
影响因子：
5.9
作者：
Fergus C;Barnes D;Alqasem MA;Kelly VP
通讯作者：
Kelly VP