A technique of cervical aortic graft transplantation in mice
A technique of cervical aortic graft transplantation in mice
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DOI：
10.1016/s1053-2498(00)00219-9
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发表时间：
2001-06-01
影响因子：
8.9
通讯作者：
Yasui, H
中科院分区：
文献类型：
--
作者：
Tomita, Y;Zhang, QW;Yasui, H
应用袖套法建立了一种小鼠颈动脉移植的新方法。用小的Teflon袖带(外径0.6 mm，内径0.4 mm)和超细尖钳分离供体的降主动脉，并用袖带技术改进小鼠异位心脏移植的方法，将供体降主动脉移植到受体小鼠的颈总动脉内。组织学分析显示，植入100天以上的同种异体移植物既没有组织损伤的证据，也没有内膜增厚的证据。我们强烈建议，这种新的小鼠主动脉移植模型是一种简单实用的血管移植研究技术。
A new method of mouse aortic graft transplantation into carotid artery was developed with cuff technique. By harvesting the descending aorta of the donor using a small Teflon cuff (external diameter 0.6 mm, internal diameter 0.4 mm) and super fine-tip forceps, and modifying the method of mouse heterotopic heart transplantation with cuff technique, donor descending aortic allografts could be interposed in the common carotid artery of recipient mice. Histological analysis demonstrated neither evidence of tissue damage nor intimal thickening in isograft implanted over 100 days. We strongly recommend that this new model of aortic transplantation in mice is a simple and useful technique for vascular transplantation research.