THE RELATIONSHIP BETWEEN LIPOGENIC ACTIVITY AND THE SIZE OF ADIPOCYTES FROM SUBCUTANEOUS ADIPOSE TISSUE OF HOLSTEIN STEERS DURING THE FATTENING PERIOD
THE RELATIONSHIP BETWEEN LIPOGENIC ACTIVITY AND THE SIZE OF ADIPOCYTES FROM SUBCUTANEOUS ADIPOSE TISSUE OF HOLSTEIN STEERS DURING THE FATTENING PERIOD
复制标题
荷斯坦牛育肥期脂肪生成活性与皮下脂肪组织脂肪细胞大小的关系
DOI：
10.2508/chikusan.69.424
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
H. Hidari
中科院分区：
文献类型：
--
作者：
M. He;S. Roh;Etsuo Shibasaki;S. Hidaka;N. Matsunaga;H. Hidari
分离的脂肪细胞用于评估不同育肥期荷斯坦阉牛皮下脂肪组织的脂肪生成活性。育肥期间，脂肪细胞的平均直径和脂肪生成量不断增加。脂肪细胞的平均直径与葡萄糖（r=0.74，P<0.05）或乙酸盐（r=0.81，P<0.05）的脂肪生成呈正相关。此外，还研究了脂肪细胞的大小和脂肪生成活性之间的关系。这可以通过测量由网格分隔的每一部分脂肪细胞中底物与脂质的结合率来完成。结果表明，育肥后期公牛脂肪细胞中葡萄糖或乙酸盐掺入率以及乙酸盐对葡萄糖掺入率的相对贡献率均显着大于早期育肥公牛（P<0.05）。在早期育肥公牛的脂肪细胞中，从级分1（33μm）到级分6（139μm），随着平均直径的增加，葡萄糖或乙酸盐掺入脂质的比率变大（两者：r=0.72，P<0.05）。育肥育肥完牛脂肪细胞中，葡萄糖掺入脂质的比率随着平均直径从级分1（36μm）到级分6（164μm）的增加而增加（r=0.79，P<0.05），而乙酸盐掺入脂质的比率仅随着平均直径从级分1（36μm）到级分4（116μm）的增加而增加，然后下降随着平均直径从部分 4 (116μm) 到部分 6 中那些非常大的脂肪细胞 (164μm) 的增加。动画。科学。技术。 （日本）69（5）：424-432，1998
Isolated adipocytes were used to evaluate the lipogenic activity in subcutaneous adipose tissue of Holstein steers during various fattening periods. During the fattening period, both the mean diameter of adipocytes and the lipogenesis increased continually. There was a positive correlation between the mean diameter of adipocytes and the lipogenesis from glucose (r =0 .74, P<0.05) or from acetate (r=0.81, P<0.05). Further, the relationship between the size of adipocyte and the lipogenic activity was investigated. It can be done through measuring the incorporation rate of substrates into lipids in each fraction of adipocytes which were separated by meshes. It was shown that both the incorporation rate of glucose or acetate into lipids and the relative contribution ratio of incorporation rate of acetate to glucose in the adipocytes taken from finishing steers, were significantly larger (P<0.05) than those from the early fattening steers. In the adipocytes which were taken from early fattening steers, the incorporation rate of glucose or acetate into lipids became larger with increment of the mean diameter from fraction 1 (33μm) to fraction 6 (139μm) (both: r=0.72, P<0.05). In the adipocytes which were taken from finishing fattening steers, the incorporation rate of glucose into lipids increased with the increment of the mean diameter from fraction 1 (36μm) to fraction 6 (164μm) (r=0.79, P<0.05), while the incorporation rate of acetate into lipids increased only with increment of the mean diameter from fraction 1 (36μm) to fraction 4 (116μm), then decreased with the increment of the mean diameter from fraction 4( 116μm) to those very large adipocytes in fraction 6 (164μm). Anim. Sci. Technol. (Jpn.) 69 (5): 424-432, 1998