Intranasal immunization with phosphorylcholine induces antigen specific mucosal and systemic immune responses in mice
Intranasal immunization with phosphorylcholine induces antigen specific mucosal and systemic immune responses in mice
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DOI：
10.1016/j.vaccine.2006.10.014
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发表时间：
2007-03-30
期刊：
影响因子：
5.5
通讯作者：
Kurono, Yuichi
中科院分区：
文献类型：
--
作者：
Tanaka, Norimitsu;Fukuyama, Satoshi;Kurono, Yuichi
磷酸胆碱（PC）是包括肺炎链球菌和流感嗜血杆菌在内的多种病原体的结构组分，并且已知抗PC免疫应答保护小鼠免受侵入性细菌疾病。本研究测试了PC作为鼻内多特异性疫苗对抗上呼吸道感染的能力。BALB/c小鼠经鼻内注射PC-keyhole limpet血蓝蛋白（KLH）加霍乱毒素（CT）作为粘膜佐剂免疫后，血清中PC特异性IgM、鼻洗液和唾液中伊加以及PC特异性鼻和脾抗体产生细胞数均增加。CD 4（+）T细胞产生IL-4和IFN-γ的增强表明Th 2和Th 1型细胞的参与。PC-KLH + CT滴鼻免疫小鼠产生的唾液伊加抗体可与大多数沙门氏菌株反应。pneumoniae和H.流感。进一步证明了S. pnemetrium和H.用PC-KLH和CT鼻免疫显著增强了来自鼻道的流感。因此，鼻内接种诱导PC特异性免疫应答可能有助于预防由S. pneumoniae和H.流感。(c)2007年由Elsevier Ltd.出版
Phosphorylcholine (PC) is a structural component of a wide variety of pathogens including Streptococcus pneunioniae and Haemophilus influenzae, and anti-PC immune responses are known to protect mice against invasive bacterial diseases. The present study tested the capability of PC as an intranasal plurispecific vaccine against upper airway infections. BALB/c mice immunized with intranasal PC-keyhole limpet hemocyanin (KLH) plus cholera toxin (CT) as a mucosal adjuvant showed increased PC-specific IgM in serum, IgA in nasal wash and saliva, and numbers of PC-specific nasal and splenic antibody producing cells. Enhanced production of IL-4 and IFN-gamma by CD4(+) T cells indicated the participation of Th2- and Th1-type cells. Salivary IgA antibodies produced by intranasal immunization with PC-KLH plus CT reacted to most strains of S. pneunioniae and H. influenzae. Further we demonstrated that the clearance of S. pnemoniae and H. influenzae from the nasal tract was significantly enhanced by nasal immunization with PC-KLH and CT. Thus, intranasal vaccination to induce PC-specific immune responses might help to prevent upper airway infections caused by S. pneumoniae and H. influenzae. (c) 2007 Published by Elsevier Ltd.