The immediate-early protein IE0 of the Autographa californica nucleopolyhedrovirus is not essential for viral replication
The immediate-early protein IE0 of the Autographa californica nucleopolyhedrovirus is not essential for viral replication
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DOI：
10.1128/jvi.79.15.10077-10082.2005
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发表时间：
2005-08-01
影响因子：
5.4
通讯作者：
Chejanovsky, N
中科院分区：
文献类型：
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作者：
Lu, LQ;Rivkin, H;Chejanovsky, N
苜蓿银纹夜蛾多重核多角体病毒 (AcMNPV) 立即早期蛋白 IE0 在杆状病毒感染中的作用尚不清楚。在本研究中，我们通过定向诱变，用 cat 基因替换外显子 0，构建了重组病毒 vAc Delta ie0 null 来表达 ie0。我们发现 vAc Delta ie0 在 Spodoptera littoralis SL2 细胞中有效复制，而该细胞对 AcMNPV 的耐受性较差。相比之下，在完全允许AcMNPV的草地贪夜蛾SF9细胞中，vAc Delta ie0 DNA复制和出芽病毒产生被延迟。这些结果和最近发表的数据（X.Dai等人，J.Virol.78：9633-9644，2004）表明ie0对于AcMNPV复制不是必需的，但在许可的SF9细胞中增强它。
The role of the Autographa californica multiple nucleopolyhedrovirus (AcMNPV) immediate-early protein IE0 in the baculloviral infection is not clear. In this study, we constructed the recombinant virus vAc Delta ie0 null for ie0 expression by targeted mutagenesis replacing exon0 with the cat gene. We found that vAc Delta ie0 replicated efficiently in Spodoptera littoralis SL2 cells, which are poorly permissive for AcMNPV. In contrast, in Spodoptera frugiperda SF9 cells, which are fully permissive for AcMNPV, vAc Delta ie0 DNA replication and budded virus production were delayed. These results and recently published data (X. Dai et al., J. Virol. 78:9633-9644,2004) indicate that ie0 is not essential for AcMNPV replication but enhances it in permissive SF9 cells.