Analysis of sucrose-induced small RNAs in Streptococcus mutans in the presence of different sucrose concentrations
Analysis of sucrose-induced small RNAs in Streptococcus mutans in the presence of different sucrose concentrations
复制标题
不同蔗糖浓度下蔗糖诱导的变形链球菌小 RNA 分析
DOI：
10.1007/s00253-017-8346-x
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发表时间：
2017-07-01
影响因子：
5
通讯作者：
Lin, Huan Cai
中科院分区：
文献类型：
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作者：
Liu, Shan Shan;Zhu, Wen Hui;Lin, Huan Cai
Streptococcus mutans (S. mutans) is the major pathogen contributing to dental caries. Sucrose is an important carbohydrate source for S. mutans and is crucial for dental caries. Small RNAs (sRNAs) are key post-transcriptional regulators of stress adaptation and virulence in bacteria. Here, for the first time, we created three replicate RNA libraries exposed to either 1 or 5% sucrose. The expression levels of sRNAs and target genes (gtfB, gtfC, and spaP) related to virulence were assessed. In addition, some phenotypic traits were evaluated. We obtained 2125 sRNA candidates with at least 100 average reads in 1% sucrose or 5% sucrose. Of these candidates, 2 were upregulated and 20 were downregulated in 1% sucrose. Six of these 22 differentially expressed sRNAs were validated by qRT-PCR. The expression level of target gene gtfB was higher in 1% sucrose. The adherence ratio of S. mutans was higher in 1% sucrose than in 5% sucrose. The synthesis of water-insoluble glucans (WIGs) was significantly higher in 5% sucrose than in 1% sucrose. These data suggest that a series of sRNAs can be induced in response to sucrose, and that some sRNAs might be involved in the regulation of phenotypes, providing new insight into the prevention of caries.
