ACETYLCHOLINESTERASE - HISTOCHEMICAL DIFFERENTIATION BETWEEN MOTOR AND SENSORY NERVE FIBERS
ACETYLCHOLINESTERASE - HISTOCHEMICAL DIFFERENTIATION BETWEEN MOTOR AND SENSORY NERVE FIBERS
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DOI：
10.1016/0006-8993(73)90373-9
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发表时间：
1973-01-01
期刊：
影响因子：
2.9
通讯作者：
ZENKER, W
中科院分区：
文献类型：
--
作者：
GRUBER, H;ZENKER, W
用Karnovsky组织化学方法观察到大鼠运动和感觉脊神经根AChE活性的显著差异。使用11个前根（VR）和10个背根（DR）从节段L3和L4，我们评估的准确性与运动和感觉神经纤维可以区分组织化学染色。染色纤维的百分比随着潜伏期的延长而增加。DR和VR之间的染色纤维的百分比的差异很小，经过短时间的孵育，也经过很长的孵育期。在孵育20-26 h时差异最大。这个时期被称为运动和感觉神经纤维分化的“关键潜伏期”。差异从未达到100%。即使在“临界孵育”后，17.7%的VR纤维仍未染色，而8.9%的DR纤维染色。这种染色的重叠在一定程度上降低了运动和感觉轴突之间的区分的准确性。交感神经节前纤维显示VR纤维的最高AChE活性，梭运动纤维的活性略低，Aα纤维的活性更低。
Marked differences in the AChE activity of rat motor and sensory spinal roots were consistently observed by means of the histochemical method of Karnovsky. Using 11 ventral roots (VR) and 10 dorsal roots (DR) from the segments L3and L4, we assessed the accuracy with which motor and sensory nerve fibres may be distinguished by histochemical staining. The percentage of stained fibres increased as the incubation period was lengthened. The difference in the percentage of stained fibres between DR and VR was small after short incubations and also after very long incubation periods. The maximal difference was found at about 20–26 h of incubation. This period is called ‘the critical incubation period’ for the differentiation between motor and sensory nerve fibres. The difference never reached 100%. Even after ‘critical incubation’ 17.7% of VR fibres were still unstained, whereas 8.9% of DR fibres were stained. This overlap of staining to some extent reduces the exactness of differentiation between motor and sensory axons.Sympathetic preganglionic fibres show the highest AChE activity of VR fibres, little less activity is present in fusimotor fibres, and still lower activity is characteristic of Aα fibres.