Novel splice variants of pro state-specific antigen and applications in diagnosis of prostate cancer
Novel splice variants of pro state-specific antigen and applications in diagnosis of prostate cancer
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DOI：
10.1016/j.clinbiochem.2007.12.022
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发表时间：
2008-05-01
影响因子：
2.8
通讯作者：
Sotiropoulou, Georgia
中科院分区：
文献类型：
--
作者：
Pampalakis, Georgios;Scorilas, Andreas;Sotiropoulou, Georgia
目的：我们的目的是确定新的剪接变异体的前列腺特异性抗原/或人激肽释放酶3（PSA/KLK 3），最广泛使用的血清生物标志物的情况下发现，筛选和监测prostate cancer.Design和方法：拼接变异体的全长序列组装成重叠群从人类EST显示同源性的cDNA序列编码PSA。通过半定量PT-PCR.Results：EST数据库挖掘的变体在临床样本中的表达进行了分析，导致识别7个以前未识别的剪接变体编码PSA样蛋白，预测含有PSA特异性抗体识别的表位序列，因此，这些异构体的表达可能会影响总PSA的量测定建立免疫分析。PSA-SV 5在前列腺增生和前列腺癌患者中的差异表达谱的分析表明，它是在前列腺癌中特异性表达的。结论：PSA的一种新的剪接变异体被鉴定，PSA-SV 5，这可能是利用在临床诊断中区分前列腺癌和前列腺增生。(c)2008年加拿大临床化学家协会。由爱思唯尔公司出版所有的紧身衣保留。
Objectives: We aimed to identify novel splice variants of prostate-specific antigen/or human kallikrein 3 (PSA/KLK3), the most widely used serum biomarker for case-finding, screening and monitoring of prostate cancer.Design and methods: The full-length sequences of splice variants were assembled as contigs from human ESTs that displayed homology to the cDNA sequence encoding PSA. Expression of variants in clinical samples was analyzed by semi-quantitative PT-PCR.Results: EST database mining led to the identification of seven previously unidentified splice variants encoding PSA-like proteins that are predicted to contain epitope sequences recognized by PSA-specific antibodies, therefore, expression of these isoforms may affect the amount of total PSA measured by established immunoassays. Analysis of the differential expression profile of isoform PSA-SV5 in patients with benign prostate hyperplasia and prostate cancer showed that it is specifically expressed in prostate cancers.Conclusions: A novel splice variant of PSA was identified, PSA-SV5, that may be exploited in clinical diagnosis to distinguish prostate cancer from benign prostate hyperplasia. (c) 2008 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All tights reserved.