Environmental determinants of the occurrence and distribution of Vibrio parahaemolyticus in the rias of Galicia, Spain
Environmental determinants of the occurrence and distribution of Vibrio parahaemolyticus in the rias of Galicia, Spain
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DOI：
10.1128/aem.01307-07
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发表时间：
2008-01-01
影响因子：
4.4
通讯作者：
Garcia-Martin, Oscar
中科院分区：
文献类型：
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作者：
Martinez-Urtaza, Jaime;Lozano-Leon, Antonio;Garcia-Martin, Oscar
据报告，加利西亚海岸（西班牙西北部）的副溶血性弧菌感染与1999年和2000年期间的大规模疾病暴发有关。关于影响这一温带地区出现副溶血性弧菌感染的生态因素的信息很少。我们进行了一项为期3年的研究，调查副溶血性弧菌的发生和分布在26个地点位于加利西亚的四个主要rias与环境和海洋学变量。在研究的所有区域和整个研究期间均检测到副溶血性弧菌，总发病率为12.5%。盐度是主要因素的时间和空间分布的副溶血性弧菌，而海水温度有次要的影响，只调制的丰富的时期和地区的盐度降低。秋季盐度较低时副溶血性弧菌的检出率较高，共有61个阳性样本（18%），平均密度为1，234个最可能数/100 g。副溶血性弧菌主要在靠近淡水排放点的盐度降低的地区检测到，在那里发现的样本高达45%。对从研究中获得的分离株进行表征，首次鉴定出从加利西亚海洋环境中回收的两种致病性tdh-positive副溶血性弧菌菌株。这些分离株的血清型相同，DNA图谱与过去10年在西班牙感染中占主导地位的副溶血性弧菌临床克隆的血清型相同。
Infections associated with Vibrio parahaemolyticus on the coast of Galicia (in northwestern Spain) were reported to be linked to large outbreaks of illness during 1999 and 2000. Little information is available about the ecological factors that influence the emergence of V. parahaemolyticus infections in this temperate region. We carried out a 3-year study to investigate the occurrence and distribution of V. parahaemolyticus at 26 sites located in the four main rias of Galicia in association with environmental and oceanographic variables. V parahaemolyticus was detected in all the areas investigated and throughout the complete period of study with an overall incidence of 12.5%. Salinity was the primary factor governing the temporal and spatial distribution of V parahaemolyticus, whereas seawater temperature had a secondary effect and only modulated the abundance in periods and areas of reduced salinities. Higher occurrence of V. parahaemolyticus was observed during periods of lower salinity in autumn, with a total of 61 positive samples (18%) and a mean density of 1,234 most probable number/100 g. V. parahaemolyticus was primarily detected in areas of reduced salinity close to freshwater discharge points, where it was found in up to 45% of the samples. Characterization of the isolates obtained from the study resulted in the first identification of two pathogenic tdh-positive strains of V. parahaemolyticus recovered from the marine environment in Galicia. These isolates showed serotypes identical to and DNA profiles indistinguishable from those of the clinical clone of V parahaemolyticus dominant in infections in Spain in the last 10 years.