BROADLY NEUTRALIZING ANTIBODIES ELICITED BY THE HYPERVARIABLE NEUTRALIZING DETERMINANT OF HIV-1
BROADLY NEUTRALIZING ANTIBODIES ELICITED BY THE HYPERVARIABLE NEUTRALIZING DETERMINANT OF HIV-1
复制标题
DOI：
10.1126/science.1703322
复制
发表时间：
1990-12-14
期刊：
影响因子：
56.9
通讯作者：
MATTHEWS, TJ
中科院分区：
文献类型：
--
作者：
JAVAHERIAN, K;LANGLOIS, AJ;MATTHEWS, TJ
人类免疫缺陷病毒 (HIV)-1 的主要中和决定簇 (PND) 位于包膜蛋白的 V3 环内。该区域的肽引发的抗体能够中和不同的分离株。来自用人 T 细胞淋巴瘤病毒 (HTLV)-IIIMN PND 肽、RP142、中和的 MN 和序列趋异的 HTLV-IIIB 分离株免疫的三只动物之一的血清。来自用 13 个氨基酸 IIIB PND 肽 (RP337) 免疫的三只动物之一的血清也中和了这两种分离物。这些血清的表征表明，交叉中和抗体与两种分离株中存在的氨基酸序列 GlyPro-GlyArgAlaPhe (GPGRAF) 结合。该序列经常出现在对随机选择的 HIV-1 分离株进行分析的 PND 中。用仅含有 GPGRAF 残基的肽免疫两只兔子的血清中和了不同的分离株，包括 IIIB 和 MN。
The principal neutralizing determinant (PND) of human immunodeficiency virus (HIV)-1 resides within the V3 loop of the envelope protein. Antibodies elicited by peptides of this region were able to neutralize diverse isolates. Serum from one of three animals immunized with the human T cell lymphoma virus (HTLV)-IIIMN PND peptide, RP142, neutralized MN and the sequence-divergent HTLV-IIIB isolate. Serum from one of three animals immunized with a 13-amino acid IIIB PND peptide (RP337) also neutralized both these isolates. Characterization of these sera revealed that the cross-neutralizing antibodies bound the amino acid sequence GlyPro-GlyArgAlaPhe (GPGRAF) that is present in both isolates. This sequence is frequently found in the PNDs analyzed in randomly selected HIV-1 isolates. Sera from two rabbits immunized with a peptide containing only the GPGRAF residues neutralized divergent isolates, including IIIB and MN.