A Noncanonical Hippo Pathway Regulates Spindle Disassembly and Cytokinesis During Meiosis in Saccharomyces cerevisiae
A Noncanonical Hippo Pathway Regulates Spindle Disassembly and Cytokinesis During Meiosis in Saccharomyces cerevisiae
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DOI：
10.1534/genetics.120.303584
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发表时间：
2020-10-01
期刊：
影响因子：
3.3
通讯作者：
Huang, Linda S.
中科院分区：
文献类型：
--
作者：
Paulissen, Scott M.;Hunt, Cindy A.;Huang, Linda S.
酿酒酵母的减数分裂用于从二倍体母细胞产生单倍体酵母孢子。在减数分裂II期间，胞质分裂通过关闭孢子膜发生，孢子膜始于纺锤体极体，并生长到包围每个单倍体减数分裂产物。需要及时关闭孢子膜，它编码一个家族的GCKIII激酶。为了确定可能激活的基因，我们利用突变体的组蛋白磷酸化缺陷来筛选具有相似表型的基因，并发现具有这种表型的基因编码一种类似河马的蛋白激酶，该蛋白是有丝分裂退出网络的一部分。我们发现，这是磷酸化所需的复合体，也是及时关闭孢子膜所必需的。我们还发现，Like是减数分裂II纺锤体分解和减数分裂后期II持续释放所必需的。然而，由有丝分裂细胞下游编码的NDR-激酶复合体似乎不在纺锤体拆解、及时关闭孢子膜或持续的后期II释放中发挥作用。综上所述，我们的结果表明，有丝分裂退出网络重新连接到减数分裂II的退出，这样就取代了CDC15下游的NDR-激酶复合体。
Meiosis in the budding yeastSaccharomyces cerevisiaeis used to create haploid yeast spores from a diploid mother cell. During meiosis II, cytokinesis occurs by closure of the prospore membrane, a membrane that initiates at the spindle pole body and grows to surround each of the haploid meiotic products. Timely prospore membrane closure requires, which encodes anfamily GCKIII kinase. To identify genes that may activate, we utilized a histone phosphorylation defect ofmutants to screen for genes with a similar phenotype and found thatshared this phenotype.encodes a Hippo-like kinase that is part of the mitotic exit network. We find thatcomplexes with, thatphosphorylation requires, and thatis also required for timely prospore membrane closure. We also find that, like, is required for meiosis II spindle disassembly and sustained anaphase II release ofin meiosis. However, the NDR-kinase complex encoded by/which functions downstream ofin mitotic cells, does not appear to play a role in spindle disassembly, timely prospore membrane closure, or sustained anaphase IIrelease. Taken together, our results suggest that the mitotic exit network is rewired for exit from meiosis II, such thatreplaces the NDR-kinase complex downstream of CDC15.